Cloned (Comment) | Organism |
---|---|
ORF Tb09.211.3670, DNA and amino acid sequence determination and analysis, generation of pRND-MHT for tetracycline-regulated expression of TbRND with a C-terminal myc-His6-TAP tag in Trypanosoma brucei, expression of N-terminally GST- and His-tagged TbRND in Escherichia coli | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
D80A | site-directed mutagenesis | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | predicted mitochondrial targeting sequence present at the N-terminus of TbRND | Trypanosoma brucei | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing | ? | - |
? | |
additional information | Trypanosoma brucei 29-13 | in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q38DE2 | - |
- |
Trypanosoma brucei 29-13 | Q38DE2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST- and His-tagged TbRND from Escherichia coli by sequential affinity chromatography on glutathione and nickel affinity resins | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing | Trypanosoma brucei | ? | - |
? | |
additional information | substrate is gA6 RNA | Trypanosoma brucei | ? | - |
? | |
additional information | in vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing | Trypanosoma brucei 29-13 | ? | - |
? | |
additional information | substrate is gA6 RNA | Trypanosoma brucei 29-13 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase D | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Trypanosoma brucei |
Organism | Comment | Expression |
---|---|---|
Trypanosoma brucei | the expression of mitochondrial exoribonuclease TbRND is highly up-regulated in the insect proliferative stage of the parasite | up |
General Information | Comment | Organism |
---|---|---|
evolution | TbRND shares sequence similarity with RNase D family enzymes but differs from all reported members of this family in possessing a CCHC zinc finger domain | Trypanosoma brucei |
malfunction | TbRND depletion results in gRNA tails extended by 2-3 nucleotides on average. Second, overexpression of wild-type but not catalytically inactive TbRND results in a substantial decrease in the total gRNA population and a consequent inhibition of RNA editing. The observed effects on the gRNA population are specific as rRNAs, which are also 3'-uridylated, are unaffected by TbRND depletion or overexpression, phenotypes, overview | Trypanosoma brucei |
physiological function | TbRND functions in guide RNA metabolism in vivo, TbRND is a 3' to 5' exoribonuclease that functions highly specific to the mitochondrion of trypanosomes. In vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridine homopolymers, including the 3' oligo(U) tails of guide RNAs that provide the sequence information for RNA editing | Trypanosoma brucei |