Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
1.6 A resolution crystal structure. Hanging-drop technique was used for subsequent crystal optimizations. Each drop contains equal volumes of protein and reservoir solution. RNase D protein crystallizes in the presence of 2.02.5 M ammonium sulfate and various buffer conditions. The crystals used here are grown with a reservoir containing 2.22.3 sulfate and 0.1 M HEPES (pH 7.0). Crystals of RNase D usually appeared overnight under these conditions. Crystals grow to full size in 27 days, with a maximum size of over 1 mm in each dimension. Zn2+ -containing crystals are grown with 1 mM ZnSO4 included in the reservoir prior to drop setup. Mercury(II) derivatives are prepared by soaking RNase D crystals (without Zn2+) overnight in the mother liquor containing 5 mM HgSO4 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | enzyme is involved in structured RNA processing | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is involved in structured RNA processing | Escherichia coli | ? | - |
? |