Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli | |
HgCl2 | the enzyme is relatively insensitive to sulfhydryl reagents, increased inhibition after addition of dithiothreitol | Escherichia coli | |
NEM | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli | |
p-hydroxymercuribenzoate | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme is devoid of nucleic acid | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 6 months, enzyme retains 90% of its activity | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is sensitive to inactivation by elevated temperatures but can be protected by a variety of RNAs | Escherichia coli |
50 | - |
3-4 min, 50% loss of the activity against phosphodiesterase-treated tRNA | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | electrofocusing, pH-range 2-11 | - |
6.2 |