Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.13.2 extracted from

  • Leo, B.; Hartl, M.J.; Schweimer, K.; Mayr, F.; Woehrl, B.M.
    Insights into the structure and activity of prototype foamy virus RNase H (2012), Retrovirology, 9, 14.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta DE3 cells Human spumaretrovirus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ two Mg2+ ions are bound in the catalytic center. The purified RNase H domain is stable in the presence of Mg2+ ions Human spumaretrovirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
1 * 18000, calculated from amino acid sequence Human spumaretrovirus
19000
-
gel filtration Human spumaretrovirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O Human spumaretrovirus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Human spumaretrovirus
-
foamy virus
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography and gel filtration Human spumaretrovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O
-
Human spumaretrovirus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 18000, calculated from amino acid sequence Human spumaretrovirus

Synonyms

Synonyms Comment Organism
RNase H
-
Human spumaretrovirus