Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MIC2067(DE3) cells | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M imidazole pH 8.0, 0.2 M NaCl, 0.4 M NaH2PO4, and 1.6 M K2HPO4 | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with Ca2+ | Sulfurisphaera tokodaii | |
Na+ | LC11-RNase H1 exhibits the highest activity in the presence of 10 mM NaCl. Its activity gradually decreases as the salt concentration increases | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15600 | - |
1 * 15600, LC11-RNase H1, calculated from amino acid sequence | Sulfurisphaera tokodaii |
16000 | - |
1 * 16000, LC11-RNase H1, SDS-PAGE | Sulfurisphaera tokodaii |
18000 | - |
LC11-RNase H1, gel filtration | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | Sulfurisphaera tokodaii | - |
? | - |
? | |
DNA-RNA hybrid + H2O | Sulfurisphaera tokodaii DSM 16993 | - |
? | - |
? | |
double-stranded RNA + H2O | Sulfurisphaera tokodaii | substrate of RNase H1 | ? | - |
? | |
double-stranded RNA + H2O | Sulfurisphaera tokodaii DSM 16993 | substrate of RNase H1 | ? | - |
? | |
additional information | Sulfurisphaera tokodaii | LC11-RNase H1 does not exhibit double strand RNase activity | ? | - |
? | |
additional information | Sulfurisphaera tokodaii DSM 16993 | LC11-RNase H1 does not exhibit double strand RNase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | F9VN79 | - |
- |
Sulfurisphaera tokodaii DSM 16993 | F9VN79 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography and Superdex 200 gel filtration | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
DNA-RNA hybrid + H2O | - |
Sulfurisphaera tokodaii DSM 16993 | ? | - |
? | |
double-stranded RNA + H2O | substrate of RNase H1 | Sulfurisphaera tokodaii | ? | - |
? | |
double-stranded RNA + H2O | substrate of RNase H1 | Sulfurisphaera tokodaii DSM 16993 | ? | - |
? | |
additional information | LC11-RNase H1 does not exhibit double strand RNase activity | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | LC11-RNase H1 does not exhibit double strand RNase activity | Sulfurisphaera tokodaii DSM 16993 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 15600, LC11-RNase H1, calculated from amino acid sequence | Sulfurisphaera tokodaii |
monomer | 1 * 16000, LC11-RNase H1, SDS-PAGE | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
LC11-RNase H1 | metagenome-derived LC11-RNase H1 is a homolog of Sulfolobus tokodaii RNase H1 that lacks a C-terminal tail, which is responsible for hyperstabilization | Sulfurisphaera tokodaii |
RNase H1 | - |
Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
optimum pH for LC11-RNase H1 | Sulfurisphaera tokodaii |