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Literature summary for 3.1.13.2 extracted from

  • Tannous, E.; Kanaya, S.
    Divalent metal ion-induced folding mechanism of RNase H1 from extreme halophilic archaeon Halobacterium sp. NRC-1 (2014), PLoS ONE, 9, e109016.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3) cells Halobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ folding of Halo-RNase H1 is induced by binding of Mg2+ (10 mM) to the bi/quad-aspartate site in a low-salt condition (50 mM NaCl) Halobacterium sp.
Mn2+ folding of Halo-RNase H1 is induced by binding of Mn2+ (1 mM) to the bi/quad-aspartate site in a low-salt condition (50 mM NaCl) Halobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O Halobacterium sp.
-
 ?
-
 ?
DNA-RNA hybrid + H2O Halobacterium sp. NRC-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Halobacterium sp. Q9HSF6
-
-
Halobacterium sp. NRC-1 Q9HSF6
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography Halobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-fluorescein-labeled RNA/DNA hybrid + H2O
-
 Halobacterium sp. ?
-
 ?
5'-fluorescein-labeled RNA/DNA hybrid + H2O
-
 Halobacterium sp. NRC-1 ?
-
 ?
DNA-RNA hybrid + H2O
-
 Halobacterium sp. ?
-
 ?
DNA-RNA hybrid + H2O
-
 Halobacterium sp. NRC-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase H1
-
 Halobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58.6
-
 melting temperature in the absence of Mn2+ Halobacterium sp.
63.1
-
 melting temperature in the presence of 1 mM Mn2+ Halobacterium sp.