Protein Variants | Comment | Organism |
---|---|---|
A558V | site-directed mutagenesis, the mutation in the enzyme primer grip results in about 3fold increased viral mutation rates compared to the wild-type enzyme/virus, reduced titer | murine leukemia virus |
Q559L | site-directed mutagenesis, the mutation in the enzyme primer grip results in about 3fold increased viral mutation rates compared to the wild-type enzyme/virus | murine leukemia virus |
S557A | site-directed mutagenesis, the mutation in the enzyme primer grip results in about 3fold increased viral mutation rates compared to the wild-type enzyme/virus, reduced titer | murine leukemia virus |
T590A | site-directed mutagenesis, the mutation in the enzyme primer grip results in similar viral mutation rates compared to the wild-type enzyme/virus | murine leukemia virus |
Y586A | site-directed mutagenesis, the mutant virus is not stable and cannot be expressed, no colonies | murine leukemia virus |
Y586F | site-directed mutagenesis, the mutation in the enzyme primer grip results in 17fold increased viral mutation rates compared to the wild-type enzyme/virus | murine leukemia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | murine leukemia virus | the RNase H primer grip is important for viral replication and replication fidelity, mutational analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
murine leukemia virus | - |
i.e. MLV | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | the RNase H primer grip is important for viral replication and replication fidelity, mutational analysis, overview | murine leukemia virus | ? | - |
? | |
DNA-RNA hybrid + H2O | the RNase H catalytic efficiency and specificity is influences by the RNase H primer grip contacting the DNA primer strand and positioning the template strand near the RNase H active site, overview | murine leukemia virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is part of the viral reverse transcriptase | murine leukemia virus |
ribonuclease H | - |
murine leukemia virus |
RNase H | - |
murine leukemia virus |