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Literature summary for 3.1.13.2 extracted from

  • Li, T.K.; Barbieri, C.M.; Lin, H.C.; Rabson, A.B.; Yang, G.; Fan, Y.; Gaffney, B.L.; Jones, R.A.; Pilch, D.S.
    Drug targeting of HIV-1 RNA.DNA hybrid structures: thermodynamics of recognition and impact on reverse transcriptase-mediated ribonuclease H activity and viral replication (2004), Biochemistry, 43, 9732-9742.
    View publication on PubMed
Search for more literature for 3.1.13.2

Application

Application Comment Organism
pharmacology the enzyme is targeted by drugs via its RNA-DNA hybrid substrates, overview Human immunodeficiency virus 1

Protein Variants

Protein Variants Comment Organism
additional information mutations of the RNase H domain of reverse transcriptase result in noninfectious virus particles Human immunodeficiency virus 1

Inhibitors

Inhibitors Comment Organism Structure
RNA-DNA duplex with bound drugs two chimeric RNA-DNA duplexes mimicking intermediates of the reverse trancriptase reaction with bound 4,5-disubstituted 2-deoxystreptamine aminoglycosides, i.e. neomycin, paromomycin, and ribostamycin, inhibit specifically and competitively the RNase H cleavage reaction by 60-95% and 15-91%, respectively, overview Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and thermodynamics Human immunodeficiency virus 1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Human immunodeficiency virus 1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA-RNA hybrid + H2O Human immunodeficiency virus 1 RNA-DNA hybrid structures: thermodynamics of recognition and impact on reverse viral replication ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
 i.e. HIV-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O RNA-DNA hybrid structures: thermodynamics of recognition and impact on reverse viral replication Human immunodeficiency virus 1 ?
-
 ?
DNA-RNA hybrid + H2O RNA-DNA hybrid structures: thermodynamics of recognition and impact on reverse transcriptase-mediated RNase H activity, cleavage sites Human immunodeficiency virus 1 ?
-
 ?

Synonyms

Synonyms Comment Organism
ribonuclease H
-
 Human immunodeficiency virus 1
RNase H
-
 Human immunodeficiency virus 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Human immunodeficiency virus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Human immunodeficiency virus 1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Human immunodeficiency virus 1