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Literature summary for 3.1.13.2 extracted from
- A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase (2006), Anal. Biochem., 351, 260-265.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of HIV-1 reverse transcriptase/RNase H in Escherichia coli strain BL21(DE3) | Human immunodeficiency virus 1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-hydroxyisoquinoline-1,3(2H,4H)-dione | IC50 values determined by two different assay variants | Human immunodeficiency virus 1 |  |
| 4-(5-benzamidothiophen-2-yl)-2,4-dioxobutanoic acid | IC50 values determined by two different assay variants | Human immunodeficiency virus 1 | |
| additional information | development of a fluorescence polarization assay for screening inhibitors against the RNase H activity of HIV-1 reverse transcriptase, two assay variants, overview | Human immunodeficiency virus 1 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Human immunodeficiency virus 1 | |
| 0.0000096 | - |
18mer DNA-RNA hybrid | pH 8.0, 37°C, recombinant enzyme | Human immunodeficiency virus 1 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Human immunodeficiency virus 1 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human immunodeficiency virus 1 | - |
i.e. HIV-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant viral enzyme from Escherichia coli strain BL21(DE3) by sequential ion exchange chromatography, and gel filtration | Human immunodeficiency virus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 18mer DNA-RNA hybrid + H2O | the RNA is labeled with 6-carboxytetramethyl rhodamine at the 3' end and annealed to complementary nonlabeled DNA | Human immunodeficiency virus 1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is part of the viral reverse transcriptase | Human immunodeficiency virus 1 |
| ribonuclease H | - |
Human immunodeficiency virus 1 |
| RNase H | - |
Human immunodeficiency virus 1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Human immunodeficiency virus 1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Human immunodeficiency virus 1 |
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