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Literature summary for 3.1.13.2 extracted from

  • Sevilya, Z.; Loya, S.; Hughes, S.H.; Hizi, A.
    The ribonuclease H activity of the reverse transcriptases of human immunodeficiency viruses type 1 and type 2 is affected by the thumb subdomain of the small protein subunits (2001), J. Mol. Biol., 311, 957-971.
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme is a major target for the chemotherapy of human immunodeficiency syndrome, AIDS Human immunodeficiency virus 1
medicine the enzyme is a major target for the chemotherapy of human immunodeficiency syndrome, AIDS Human immunodeficiency virus 2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Human immunodeficiency virus 1
expression in Escherichia coli and expression in bacteria by plasmids Human immunodeficiency virus 2

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
HIV-1
-
Human immunodeficiency virus 2
-
Rod strain and D-194 strain of HIV-2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information construction of chimeric HIV-1/HIV-2 enzymes, The specific activity is either high, like HIV-1 enzyme, or low, like HIV-2 enzyme. The origin of the thumb subdomain in the small subunit of the chimeric enzymes, residues 244-322, determines the level of the activity. Human immunodeficiency virus 1
additional information construction of chimeric HIV-1/HIV-2 enzymes. The specific activity is either high, like HIV-1 enzyme, or low, like HIV-2 enzyme. The origin of the thumb subdomain in the small subunit of the chimeric enzymes, residues 244-322, determines the level of the activity. Human immunodeficiency virus 2

Purification (Commentary)

Purification (Comment) Organism
wild-type and chimeric enzymes Human immunodeficiency virus 1
wild-type and chimeric enzymes Human immunodeficiency virus 2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
comparison of relative activities of wild-type and chimeric enzymes, the enzyme activity of HIV-2 is about tenfold lower than of HIV-1 Human immunodeficiency virus 1
additional information
-
comparison of relative activities of wild-type and chimeric enzymes, the enzyme activity of HIV-2 is about tenfold lower than of HIV-1 Human immunodeficiency virus 2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5' end-labeled RNA cleavage of 267 nt RNA substrate to produce a 47 nt long product, the second cleavage produces RNA fragments that are 38 nt long Human immunodeficiency virus 1 RNA fragments
-
?
5'-end-labeled RNA cleavage of 267nt RNA substrate to produce a 47nt long product, the second cleavage produces RNA fragments that are 38nt long. Human immunodeficiency virus 2 RNA fragments
-
?
5'-end-labeled RNA cleavage of 267nt RNA substrate to produce a 47nt long product, the second cleavage produces RNA fragments that are 38nt long. Human immunodeficiency virus 2 D-194 RNA fragments
-
?
5'-end-labeled RNA cleavage of 267nt RNA substrate to produce a 47nt long product, the second cleavage produces RNA fragments that are 38nt long. Human immunodeficiency virus 2 ROD RNA fragments
-
?
poly(rA)n-poly(dT)n
-
Human immunodeficiency virus 1 ?
-
?
poly(rA)n-poly(dT)n
-
Human immunodeficiency virus 2 ?
-
?
poly(rA)n-poly(dT)n
-
Human immunodeficiency virus 2 D-194 ?
-
?
poly(rA)n-poly(dT)n
-
Human immunodeficiency virus 2 ROD ?
-
?

Subunits

Subunits Comment Organism
heterodimer p66/p51 Human immunodeficiency virus 1
heterodimer p68/p54 Human immunodeficiency virus 2