Literature summary for 3.1.13.2 extracted from

Guaitiao, J.P.; Zuniga, R.A.; Roth, M.J.; Leon, O.
Lysine directed cross-linking of viral DNA-RNA:DNA hybrid substrate to the isolated RNase H domain of HIV-1 reverse transcriptase (2004), Biochemistry, 43, 1302-1308.

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Protein Variants

Protein Variants	Comment	Organism
K476C	the mutation disrupts the chemical cross-linking while maintaining activity	Human immunodeficiency virus 1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required for the cross-linking of the modified substrate to RNase H	Human immunodeficiency virus 1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA-RNA hybrid duplex + H2O	Human immunodeficiency virus 1	-	oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety	-	?

Organism

Organism	UniProt	Comment	Textmining
Human immunodeficiency virus 1	-	HIV-1	-

Reaction

Reaction	Comment	Organism	Reaction ID
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid	two activities are present in the reverse transcriptase: polymerase and RNase H. RNase H is required for removal of the viral RNA, primer formation and removal for the synthesis of the (+) strand DNA, and specific removal of the primer tRNA used in the synthesis of the (-) strand DNA.	Human immunodeficiency virus 1	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA-RNA hybrid duplex + H2O	-	Human immunodeficiency virus 1	oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety	-	?
DNA-RNA hybrid duplex + H2O	the RNase H domain of reverse transcriptase catalyses the cleavage of the RNA within DNA-RNA hybrids, both in a polymerase-dependent or independent fashion. Polymerase-dependent: Cleavage occurring at a distance of 18-20 nucleotides behind DNA polymerization. Polymerase-independent: cleavage of the viral RNA for the initiation of second DNA strand synthesis and for removal of the primer tRNA.	Human immunodeficiency virus 1	oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety	-	?

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Release 2023.1 (January 2023)