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Literature summary for 3.1.13.1 extracted from

  • Matos, R.G.; Fialho, A.M.; Giloh, M.; Schuster, G.; Arraiano, C.M.
    The rnb gene of Synechocystis PCC6803 encodes a RNA hydrolase displaying RNase II and not RNase R enzymatic properties (2012), PLoS ONE, 7, e32690.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ may partly substitute for Mg2+, with less efficiency Synechocystis sp.
KCl preferred concentration is 50 mM. Activity starts to decrease above 150 mM Synechocystis sp.
Mg2+ required for activity, preferred concntration is 1 mM Synechocystis sp.
NaCl enzyme is active in NaCl, but the cleavage efficiency is 10times lower when compared to its activity in KCl Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsRNA + H2O
-
Synechocystis sp. ?
-
?
additional information enzyme has no preference for poly(A) or randomized sequenced RNA Synechocystis sp. ?
-
?
petD3 RNA + H2O
-
Synechocystis sp. 5'-phosphomononucleotides products detected are exclusively nucleotide monophosphates ?
poly(A) + H2O
-
Synechocystis sp. ? in presence of 50 nM of protein, the final product released is a 5 nt fragment.When a higher protein concentration, 250 nM, is used the protein is able to release a 4 nt end-product ?
ssRNA + H2O
-
Synechocystis sp. ? release of a 4 nt fragment ?

Synonyms

Synonyms Comment Organism
Rnb
-
Synechocystis sp.

General Information

General Information Comment Organism
physiological function RNase II protein is essential for vialbilty Synechocystis sp.