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Literature summary for 3.1.13.1 extracted from

  • Nossal, N.G.; Singer, M.F.
    The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II (1968), J. Biol. Chem., 243, 913-922.
    View publication on PubMed
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Metals/Ions

Metals/Ions Comment Organism Structure
K+ activating Escherichia coli
Mg2+ necessary for activity Escherichia coli
NH4+ activating Escherichia coli
NH4+ can substitute for K+ to a small extend Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 sucrose density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ss RNA + H2O Escherichia coli
-
 5'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information helical RNA is not hydrolyzed Escherichia coli ?
-
 ?
poly(A) + H2O
-
 Escherichia coli 5'-AMP + oligonucleotide
-
 ?
ss RNA + H2O
-
 Escherichia coli 5'-phosphomononucleotides
-
 ?
ss RNA oligonucleotides with chain lengths less than seven + H2O at high concentrations Escherichia coli 5'-phosphomononucleotides
-
 ?
tRNA + H2O poor substrate Escherichia coli ?
-
 ?