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Literature summary for 3.1.12.1 extracted from

  • Zhang, J.; Kasciukovic, T.; White, M.F.
    The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron-sulfur cluster (2012), PLoS One, 7, e47232.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.12.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Posetta DE3 Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D99A the mutant enzyme fails to cut DNA Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
iron-sulfur centre contains a [4Fe-4S] cluster Saccharolobus solfataricus
Mg2+ cleavage of a 15T DNA oligonucleotide by Sso0001 is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium Saccharolobus solfataricus
Mn2+ cleavage of a 15T DNA oligonucleotide by Sso0001 is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23239
-
 x * 23239, calculated from sequence Saccharolobus solfataricus
170000
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97TX9
-
-
Saccharolobus solfataricus P2 Q97TX9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-80°C, stable Saccharolobus solfataricus
4°C, enzyme loses colour and activity over several days Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded oligodeoxyribonucleotide + H2O cleavage of a 15T DNA oligonucleotide is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium. Saccharolobus solfataricus nucleoside 3'-phosphate + ?
-
 ?
single-stranded oligodeoxyribonucleotide + H2O cleavage of a 15T DNA oligonucleotide is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 1–5 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium. Saccharolobus solfataricus P2 nucleoside 3'-phosphate + ?
-
 ?
ssDNA + H2O the enzyme is specific for ssDNA Saccharolobus solfataricus nucleoside 3'-phosphate + ?
-
 ?
ssDNA + H2O the enzyme is specific for ssDNA Saccharolobus solfataricus P2 nucleoside 3'-phosphate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 23239, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
5' to 3' single stranded DNA exonuclease
-
 Saccharolobus solfataricus
Cas4
-
 Saccharolobus solfataricus
SSO0001 locus name Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function the role of the enzyme in vivo may be to process viral DNA to generate new spacers with 3' ssDNA overhangs suitable for recombination or integration in a CRISPR (Clusters of Regularly interspaced Palindromic Repeats) locus mediated by the enzyme Cas1 Saccharolobus solfataricus