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Literature summary for 3.1.11.6 extracted from

  • Chase, J.W.; Vales, L.D.
    Exonuclease VII of E. coli (1981), Gene Amplif. Anal., 2, 147-168.
    View publication on PubMed

Application

Application Comment Organism
analysis degradation of adenovirus-associated DNA Escherichia coli
analysis of nucleic acid structure Escherichia coli
analysis removal of single-stranded DNA Escherichia coli
analysis of spliced RNA Escherichia coli
analysis excision of DNA segments from cloning vectors Escherichia coli

General Stability

General Stability Organism
freezing and thawing causes loss of activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
single-stranded DNA + H2O Escherichia coli
-
hydrolyzed single-stranded DNA oligonucleotides ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
xseA
-
Escherichia coli
-
numerous mutants
-

Purification (Commentary)

Purification (Comment) Organism
1700fold Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 20 mM Tris-HCl, pH 8, 10 mM mercaptoethanol, 0.1 mM EDTA, 0.2 M NaCl, 50% glycerol, 18 months, 10% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded DNA + H2O
-
Escherichia coli hydrolyzed single-stranded DNA oligonucleotides ?
single-stranded DNA + H2O processive degradation Escherichia coli hydrolyzed single-stranded DNA
-
?
thymine dimers + H2O possible role in repair of ultraviolet damage Escherichia coli hydrolyzed single-stranded DNA
-
?