Application | Comment | Organism |
---|---|---|
analysis | degradation of adenovirus-associated DNA | Escherichia coli |
analysis | of nucleic acid structure | Escherichia coli |
analysis | removal of single-stranded DNA | Escherichia coli |
analysis | of spliced RNA | Escherichia coli |
analysis | excision of DNA segments from cloning vectors | Escherichia coli |
General Stability | Organism |
---|---|
freezing and thawing causes loss of activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
single-stranded DNA + H2O | Escherichia coli | - |
hydrolyzed single-stranded DNA | oligonucleotides | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
xseA | - |
Escherichia coli | - |
numerous mutants | - |
Purification (Comment) | Organism |
---|---|
1700fold | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl, pH 8, 10 mM mercaptoethanol, 0.1 mM EDTA, 0.2 M NaCl, 50% glycerol, 18 months, 10% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
single-stranded DNA + H2O | - |
Escherichia coli | hydrolyzed single-stranded DNA | oligonucleotides | ? | |
single-stranded DNA + H2O | processive degradation | Escherichia coli | hydrolyzed single-stranded DNA | - |
? | |
thymine dimers + H2O | possible role in repair of ultraviolet damage | Escherichia coli | hydrolyzed single-stranded DNA | - |
? |