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Literature summary for 3.1.11.5 extracted from
- The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination, and DNA repair (2000), Proc. Natl. Acad. Sci. USA, 97, 7399-7404.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D1080A | Comparison: recB(D1080A)CD is recombinant-deficient, and sensitive to DNA damaging agents, and the purified enzyme failed to load RecA during DNA winding. recB(D1080A)C is recombinant-proficient and resistant to DNA damaging agents, and the mutant is active RecA loading assay. | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutant enzyme | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides | The enzyme is required for homologous recombination and DNA repair, the degradative and recombinational activities of enzyme, as well as its structure, are regulated by a specific DNA sequence called Chi. The recombination requires loading of RecA by RecBCD enzyme and that the RecD subunit inhibits this reaction. | Escherichia coli |
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