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Literature summary for 3.1.11.5 extracted from
- Processive translocation and DNA unwinding by individual RecBCD enzyme molecules (2001), Nature, 409, 374-378.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA | Escherichia coli | ATP-dependent, unwinding of DNA molecule | single-stranded DNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA | ATP-dependent, unwinding of DNA molecule | Escherichia coli | single-stranded DNA | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 37 | The rate of unwinding in 1 mM ATP increases twofold when the temperature is raised from 23°C to 37°C. | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | The corrected unwinding rate is 443 base pairs 1/sec, which is due to a single molecule of enzyme that bound to the free double-stranded DNA end opposite the bead, and both translocates and unwinds the DNA in an ATP-dependent manner once the DNA entered the ATP channel. The rate of unwinding increases with increasing ATP concentration and increasing temperature | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | the unwinding of | Escherichia coli | |
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Release 2023.1 (January 2023)
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