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Literature summary for 3.1.11.5 extracted from

  • Chedin, F.; Kowalczykowski, S.C.
    A novel family of regulated helicases/nucleases from Gram-positive bacteria: insights into the initiation of DNA recombination (2002), Mol. Microbiol., 43, 823-834.
    View publication on PubMed
Search for more literature for 3.1.11.5

Application

Application Comment Organism
additional information AddA mutants that cannot bind or hydrolyze ATP are completely defective for DNA recombination Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
addA and addB Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information RecB mutants that cannot bind or hydrolyze ATP are completely defective for DNA recombination Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 1 * 135000 + 1 * 141000, addA and addB gene encode two subunits of enzyme, the enzyme carries two homologous nuclease domains Bacillus subtilis
141000
-
 1 * 135000 + 1 * 141000, addA and addB gene encode two subunits of enzyme, the enzyme carries two homologous nuclease domains Bacillus subtilis
330000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA Bacillus subtilis the enzyme plays a an important role in the initiation of DNA recombination and recombinant-dependent DNA replication single-stranded DNA fragments
-
 ?
double-stranded DNA Escherichia coli the enzyme plays a an important role in the initiation of DNA recombination and recombinant-dependent DNA replication single-stranded DNA fragments
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides The enzyme is involved initiating DNA recombination. The nuclease properties of the enzyme are regulated upon recognition of a specific DNA sequence by the translocating enzyme. This sequence, called chi, corresponds to the octamer 5’-GCTGGTGG-3’. Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA ATP dependent double-stranded DNA exonuclease and processive DNA helicase Escherichia coli single-stranded DNA fragments
-
 ?
double-stranded DNA the enzyme is a powerful and processive DNA helicase, a potent double-stranded DNA exonuclease and an ATPase, the enzyme is regulated by a Chi cognate sequence, the degradation of DNA by enzyme is nearly symmetrical on the both strands Bacillus subtilis single-stranded DNA fragments
-
 ?
double-stranded DNA the enzyme plays a an important role in the initiation of DNA recombination and recombinant-dependent DNA replication Bacillus subtilis single-stranded DNA fragments
-
 ?
double-stranded DNA the enzyme plays a an important role in the initiation of DNA recombination and recombinant-dependent DNA replication Escherichia coli single-stranded DNA fragments
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 135000 + 1 * 141000, addA and addB gene encode two subunits of enzyme, the enzyme carries two homologous nuclease domains Bacillus subtilis
heterotrimer complex of three RecB, RecC, RecD proteins Escherichia coli
More the difference between the AddAB and RecBCD enzymes is that the two enzyme have a very different subunit composition, the AddA subunit is a direct homologue of the RecB subunit, the helicases of each complex Bacillus subtilis

Synonyms

Synonyms Comment Organism
AddAB enzyme
-
 Bacillus subtilis
ExoV
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP required Bacillus subtilis