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Literature summary for 3.1.11.5 extracted from

  • Amundsen, S.K.; Taylor, A.F.; Smith, G.R.
    A domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli RecBCD holoenzyme (2002), Genetics, 161, 483-492.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.5

Cloned(Commentary)

Cloned (Comment) Organism
recC gene Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information recBC1010D, recBC1041D and recBCD1013 mutants have less than 0.2% of the exonuclease activity of wild-type enzyme. recBC1010D and recBC1041D produce RecD but fail to assemble it into holoenzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
douple-stranded DNA Escherichia coli the enzyme is required for the major pathway of double-strand DNA break repair and genetic exchange, the enzyme has potent nuclease and helicase activity ?
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-

Purification (Commentary)

Purification (Comment) Organism
RecC, RecBC and RecBCD, RecBC1041 mutant Escherichia coli

Storage Stability

Storage Stability Organism
4°C, RecC, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
douple-stranded DNA the enzyme has potent nuclease and helicase activity Escherichia coli ?
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 ?
douple-stranded DNA the enzyme is required for the major pathway of double-strand DNA break repair and genetic exchange, the enzyme has potent nuclease and helicase activity Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
heterotrimer RecB, RecC and RecD, The C-terminus of RecC: 35000 Da, is required for assembly of the RecD subunit into enzyme holoenzyme but not for recombination proficiency. The N-terminus of RecC: 95000 Da, contains functions essential for recombination and is also required for chi activity. RecD is essential for nuclease activity, regulation by the recombination hotspot Chi, and high affinity for DNA ends. RecC interacts with RecD to form a heterotrimer only in the presence of RecB. Escherichia coli