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Literature summary for 3.1.11.4 extracted from

  • Trilling, D.M.; Aposhian, H.V.
    Sequential cleavage of dinucleotides from DNA by phage SP3 DNase (1968), Proc. Natl. Acad. Sci. USA, 60, 214-221.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.4

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activity, no activity in less than 6.7 mM Enterobacteria phage SP
Mg2+ optimum for activation between 13 and 25 mM Enterobacteria phage SP
Mn2+ at 1.3 mM 44% of activation by Mg2+ Enterobacteria phage SP

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
single-stranded DNA + H2O Enterobacteria phage SP
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Enterobacteria phage SP
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, phosphocellulose, sucrose gradient sedimentation Enterobacteria phage SP

Source Tissue

Source Tissue Comment Organism Textmining
infected cell infected Bacillus subtilis Enterobacteria phage SP
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded DNA + H2O hydrolysis from the 5-and 3-termini of single-stranded DNA Enterobacteria phage SP dinucleotide + trinucleotide general structure of dinucleotide is d-pX-pY and of trinucleotide is d-pX-pY-pZ ?
single-stranded DNA + H2O
-
 Enterobacteria phage SP ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.9
-
 Enterobacteria phage SP