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Literature summary for 3.1.11.2 extracted from
- Combined micrococcal nuclease and exonuclease III digestion reveals precise positions of the nucleosome core/linker junctions: implications for high-resolution nucleosome mapping (2013), J. Mol. Biol., 425, 1946-1960.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | a combined treatment of nucleosomes with micrococcal nuclease and exonuclease III overcomes micrococcal nuclease sequence preference and produces nucleosomal DNA trimmed symmetrically and precisely at the core/linker junctions regardless of the underlying DNA sequence. Combined micrococcal nuclease/exonuclease III digestion can be applied to in situ chromatin for unbiased genome-wide mapping of nucleosome positions that is not ifluenced by DNA sequences at the core/linker junctions. The same approach can be also used for the precise mapping of the extent of linker DNA protection by H1 and other protein factors associated with nucleosome linkers | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Escherichia coli | - |
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Release 2023.1 (January 2023)
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