Any feedback?
Literature summary for 3.1.11.2 extracted from
- Sensitive and selective amplified fluorescence DNA detection based on exonuclease III-aided target recycling (2010), J. Am. Chem. Soc., 132, 1816-1818.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | using the cleavage function of exonuclease III on double-stranded DNA, design of an amplified DNA detection scheme employing a stem-loop DNA molecular beacon as the signaling probe. Exonuclease III used to recycle target molecules, thus leading to improved sensitivity relative to traditional molecular beacons without any significant restriction in the choice of target sequences | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | exonuclease III does not require a specific recognition site, so cleavage occurs irrespective of the sequence present at the blunt terminus. Amplification provided by exonuclease III leads to a 10fold increase in the final fluorescence intensity observed at 37°C upon addition of 0.001 mM target | Escherichia coli | deoxynucleoside 5'-phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ExoIII | - |
Escherichia coli |
| exonuclease III | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
