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Literature summary for 3.1.11.2 extracted from

  • Burkovics, P.; Szukacsov, V.; Unk, I.; Haracska, L.
    Human Ape2 protein has a 3-5 exonuclease activity that acts preferentially on mismatched base pairs (2006), Nucleic Acids Res., 34, 2508-2515.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.2

Cloned(Commentary)

Cloned (Comment) Organism
into the Gateway entry vector pENTR3C, for expressing Ape2 proteins in the yeast strain BJ5464, the cDNAs are cloned in fusion with GST using the plasmid pBJ842 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D277A mutant lacking all enzymatic activities Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens
Mn2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 determined by SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a glutathione-Sepharose column Homo sapiens

Synonyms

Synonyms Comment Organism
Ape2 Ape2 exibits strong 3'-5' exonuclease and 3'-phosphodiesterase activities and has only a very weak AP-endonuclease activity Homo sapiens
exodeoxyribonuclease III
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 3'-5' exonuclease, 3'-phosphodiesterase and AP-endonuclease activity assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 3'-5' exonuclease, 3'-phosphodiesterase and AP-endonuclease activity assay Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Homo sapiens