Crystallization (Comment) | Organism |
---|---|
crystallization of recombinant wild-type native and selenomethionine-labeled enzyme, and of recombinant mutant D173A enzyme in complex with metal ions, X-ray diffraction structure determination and analysis at 2.5-3.4 A resolution, modelling | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D173A | inactive mutant, structure in complex with Ca2+, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the K+-binding site lies in the H2TH domain | Homo sapiens | |
Mg2+ | required | Homo sapiens | |
additional information | wild-type enzyme structures in complex with Mn2+ or Ba2+, binding structures, anomalous scattering, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UQ84 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate is radiolabeled 5' recessed DNA, interactions between the hExo1 protein and DNA substrate, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme structure analysis, including pre-nick and post-nick DNA-binding region, and the active site | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Exo1 | - |
Homo sapiens |
exonuclease 1 | - |
Homo sapiens |
More | Exo1 is a member of the 5' structure-specific nuclease family of exonucleases and endonucleases that includes FEN-1, XPG, and GEN1 | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | structures of hExo1 in complex with a DNA substrate, overview | Homo sapiens |
physiological function | human exonuclease 1 plays important roles in DNA repair and recombination processes that maintain genomic integrity, model of 5' exonuclease activity in human DNA mismatch repair, overview. Analysis of the mechanism by which this 5' structure-specific exo- and endonuclease family, including Exo1, recognizes and processes diverse DNA structures, conformational control of a mobile region in the catalytic site suggests a mechanism for allosteric regulation by binding to protein partners, overview | Homo sapiens |