Literature summary for 3.1.11.1 extracted from

Nimonkar, A.V.; Ozsoy, A.Z.; Genschel, J.; Modrich, P.; Kowalczykowski, S.C.
Human exonuclease 1 and BLM helicase interact to resect DNA and initiate DNA repair (2008), Proc. Natl. Acad. Sci. USA, 105, 16906-16911.

Search for more literature for 3.1.11.1

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	human BLM helicase, a member of the RecQ family, stimulates the nucleolytic activity of Exo1. Stimulation of hExo1 by BLM does not require ATP. Stimulation is specific because other RecQ homologs (human WRN, RecQ1, RecQ4 and RecQ5beta and Escherichia coli RecQ) fail to stimulate Exo1. RPA does not affect the nuclease activity of Exo1 alone, nor does it appreciably change substrate utilization by Exo1 in the presence of BLM helicase. BLM helicase physically interacts with Exo1	Homo sapiens

Application

Application	Comment	Organism
additional information	role of BLM and Exo1 in the initiation of recombinational DNA repair. Stimulation of DNA resection by human Exo1 is independent of BLM helicase activity and is, instead, mediated by an interaction between the two proteins. DNA ends resected by Exo1 and BLM are used by human Rad51, but not its yeast or bacterial counterparts, to promote homologous DNA pairing	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D173A	with either mutant D173A alone or both mutant D173A and BLM, nucleolytic degradation is undetectable. It competitively inhibits Exo1-BLM-mediated resection	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	Escherichia coli RecQ inhibits Exo1, most likely by blocking Exo1 access to the ends of DNA	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-end-labeled dsDNA + H2O	-	Homo sapiens	?	-	?
5'-end-labeled dsDNA + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
Exo1	-	Homo sapiens
exonuclease 1	-	Homo sapiens

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Release 2023.1 (January 2023)