Any feedback?
Literature summary for 3.1.1.8 extracted from
- Increased organophosphate scavenging in a butyrylcholinesterase mutant (2008), Chem. Biol. Interact., 175, 376-379.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of a A328W/Y332A mutant BChE with improved cocaine hydrolase activity in transgenic Nicotiana benthamiana plants | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A328W/Y332A | the engineered recombinant cocaine hydrolase displays increased cocaine hydrolysis ability and increased affinity to the organophosphate pesticides paraoxon and malaoxon compared to the wild-type enzyme, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chlorpyrifos-oxon | - |
Homo sapiens |  |
| diisopropylfluorophosphate | - |
Homo sapiens | |
| malaoxon | - |
Homo sapiens | |
| additional information | the engineered recombinant BChE mutant A328W/Y332A displays increased affinity to the organophosphate pesticides paraoxon and malaoxon compared to the wild-type enzyme, overview | Homo sapiens | |
| neostigmine bromide | - |
Homo sapiens | |
| paraoxon | - |
Homo sapiens | |
| tetra(monoisopropyl) diphosphortetramide | i.e. iso-OMPA | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
cocaine | A328W/Y332A mutant BChE | Homo sapiens | |
| 0.434 | - |
cocaine | wild-type BChE | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cocaine + H2O | Homo sapiens | - |
ecgonine methyl ester + benzoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cocaine + H2O | - |
Homo sapiens | ecgonine methyl ester + benzoate | - |
? | |
| cocaine + H2O | cv. EC 3.1.1.1 | Homo sapiens | ecgonine methyl ester + benzoate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BChE | - |
Homo sapiens |
| butyrylcholinesterase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 386.8 | - |
cocaine | A328W/Y332A mutant BChE | Homo sapiens | |
| 418.3 | - |
cocaine | wild-type BChE | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
