Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in CHO-K1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D70G | site-directed mutagenesis, peripheral site mutant, altered kinetics compared to the wild-type enzyme and no substrate activation, overview | Homo sapiens |
S198C | site-directed mutagenesis, inactive catalytic site mutant | Homo sapiens |
S198D | site-directed mutagenesis, inactive catalytic site mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Echothiophate | an organophosphorous compound, inhibition kinetics, overview | Homo sapiens | |
additional information | mo product inhibition with aryl acetylamides | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics with aryl acetylamide substrates, overview, low affinity, first order kinetics with neutral amides | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylthiocholine + H2O | Homo sapiens | - |
thiocholine + butyrate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from CHO-K1 cells by anion exchange and affinity chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acylcholine + H2O = choline + a carboxylate | the catalytic residue is Ser198 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2,2-trifluoro-N-(2-nitrophenyl)acetamide + H2O | a neutral amide | Homo sapiens | 2-nitroaniline + trifluoroacetate | - |
? | |
2-nitroacetanilide + H2O | a neutral amide | Homo sapiens | 2-nitroaniline + acetate | - |
? | |
3-(acetamido) N,N,N-trimethylanilinium + H2O | a positively charged amide, hydrolysis by the wild-type enzyme and the mutant D70G shows a long transient phase preceeding the steady state, hysteretic burst induction phase, overview | Homo sapiens | 3-amino-N,N,N-trimethylanilinium + acetate | - |
? | |
3-nitroacetanilide + H2O | a neutral amide | Homo sapiens | 3-nitroaniline + acetate | - |
? | |
butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? | |
additional information | BChE also shows aryl acylamidase activity, i.e. AAA activity, the rate-determining step of which is the acylation, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | recombinant wild-type enzyme | Homo sapiens |
tetramer | native wild-type enzyme | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
butyrylcholinesterase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Homo sapiens |