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Literature summary for 3.1.1.8 extracted from
- DNA sequence of butyrylcholinesterase from the rat: expression of the protein and characterization of the properties of rat butyrylcholinesterase (2002), Biochem. Pharmacol., 63, 2101-2110.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in CHO cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R286L | Km values similar to wild-type enzyme | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Echothiophate | enzyme spontaneously reactivates with a half-life of 4.3 h | Rattus norvegicus |  |
| Triton X-100 | competitive inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
acetylthiocholine | pH 7.0, 25°C, R286L mutant | Rattus norvegicus | |
| 0.061 | - |
acetylthiocholine | pH 7.0, 25°C, wild-type | Rattus norvegicus | |
| 0.094 | - |
butyrylthiocholine | pH 7.0, 25°C, R286L mutant | Rattus norvegicus | |
| 0.112 | - |
propionylthiocholine | pH 7.0, 25°C, R286L mutant | Rattus norvegicus | |
| 0.134 | - |
butyrylthiocholine | pH 7.0, 25°C, wild-type | Rattus norvegicus | |
| 0.189 | - |
propionylthiocholine | pH 7.0, 25°C, wild-type | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylthiocholine + H2O | - |
Rattus norvegicus | thiocholine + acetate | - |
? | |
| benzoylcholine + H2O | - |
Rattus norvegicus | choline + benzoate | - |
? | |
| bytyrylhiocholine + H2O | - |
Rattus norvegicus | thiocholine + butyrate | - |
? | |
| cocaine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| propionylthiocholine + H2O | best substrate | Rattus norvegicus | thiocholine + propionate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3350 | - |
propionylthiocholine | pH 7.0, 25°C | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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