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Literature summary for 3.1.1.73 extracted from
- Contribution of disulfide bridges to the thermostability of a type a feruloyl esterase from Aspergillus usamii (2015), PLoS ONE, 10, e0126864.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Aspergillus usamii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A126C/N152C | introduction of a disulfide bridge, increase of temperature optimum by 6°C and increase in thermal inactivation half-live | Aspergillus usamii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.62 | - |
ferulic acid 4-nitrophenyl ester | wild-type, pH 5.5, 40°C | Aspergillus usamii |  |
| 5.98 | - |
ferulic acid 4-nitrophenyl ester | mutant A126C/N152C, pH 5.5, 40°C | Aspergillus usamii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Aspergillus usamii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus usamii | V5TEY2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferulic acid 4-nitrophenyl ester + H2O | - |
Aspergillus usamii | ferulic acid + 4-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Aspergillus usamii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAEA | - |
Aspergillus usamii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
wild-type | Aspergillus usamii |
| 51 | - |
mutant A126C/N152C | Aspergillus usamii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life of wild-type 15 min, of mutant A126C/N152C 188 min, respectively | Aspergillus usamii |
| 60 | - |
half-life of wild-type 4 min, of mutant A126C/N152C 40 min, respectively | Aspergillus usamii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 135 | - |
ferulic acid 4-nitrophenyl ester | wild-type, pH 5.5, 40°C | Aspergillus usamii | |
| 213 | - |
ferulic acid 4-nitrophenyl ester | mutant A126C/N152C, pH 5.5, 40°C | Aspergillus usamii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35.68 | - |
ferulic acid 4-nitrophenyl ester | mutant A126C/N152C, pH 5.5, 40°C | Aspergillus usamii | |
| 37.25 | - |
ferulic acid 4-nitrophenyl ester | wild-type, pH 5.5, 40°C | Aspergillus usamii | |
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Release 2023.1 (January 2023)
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