Protein Variants | Comment | Organism |
---|---|---|
T27I/S84L/V161I/G243C | mutant displays significantly improved thermostability | uncultured bacterium |
T27I/S84L/V161I/H195L/G243C/A259V | mutant displays significantly improved thermostability | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C | uncultured bacterium | |
0.42 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | E7DJY5 | - |
- |
Storage Stability | Organism |
---|---|
25°C, storage for 150 days, the activity of mutant T27I/S84L/V161I/H195L/G243C/A259V remains almost constant (100.5%), whereas that of the wild-type is only 21.2% | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl ferulate + H2O | - |
uncultured bacterium | ferulic acid + 4-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EstF27 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
wild-type | uncultured bacterium |
60 | - |
mutant T27I/S84L/V161I/G243C | uncultured bacterium |
65 | - |
mutant T27I/S84L/V161I/H195L/G243C/A259V | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life of wild-type 0.5 h, mutant T27I/S84L/V161I/G243C 70 h, mutant T27I/S84L/V161I/H195L/G243C/A259V 1680 h, respectively | uncultured bacterium |
65 | - |
half-life of mutant T27I/S84L/V161I/G243C 4 h, mutant T27I/S84L/V161I/H195L/G243C/A259V 140 h, respectively | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.9 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C | uncultured bacterium | |
32.2 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.4 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C | uncultured bacterium | |
76.6 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C | uncultured bacterium |