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Literature summary for 3.1.1.73 extracted from

  • Wang, L.; Ma, Z.; Du, F.; Wang, H.; Ng, T.B.
    Feruloyl esterase from the edible mushroom Panus giganteus: a potential dietary supplement (2014), J. Agric. Food Chem., 62, 7822-7827.
    View publication on PubMed
Search for more literature for 3.1.1.73

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 40% inhibition at 5 mM Pleurotus giganteus
Ca2+ 60% inhibition at 5 mM Pleurotus giganteus
Cd2+ 60% inhibition at 5 mM Pleurotus giganteus
Cu2+ 40% inhibition at 5 mM Pleurotus giganteus
K+ 40% inhibition at 5 mM Pleurotus giganteus
Mg2+ 40% inhibition at 5 mM Pleurotus giganteus
Mn2+ 60% inhibition at 5 mM Pleurotus giganteus
Pb2+ 20% inhibition at 5 mM Pleurotus giganteus
Zn2+ 40% inhibition at 5 mM Pleurotus giganteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 methyl ferulate pH 5.0, 37°C Pleurotus giganteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Pleurotus giganteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pleurotus giganteus the single purified enzyme brings about the release of ferulic acid from wheat bran and corn bran, and the amount released from wheat bran rises to 51.9% (of the total amount) by the synergistic action of xylanase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pleurotus giganteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 130.8fold from fruiting bodies by two different steps of anion exchange chromatography, and gel filtration Pleurotus giganteus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body fresh Pleurotus giganteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
170
-
 purified enzyme, pH 5.0, 37°C Pleurotus giganteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Pleurotus giganteus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Pleurotus giganteus 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caproate + H2O
-
 Pleurotus giganteus 4-nitrophenol + caproate
-
 ?
methyl 4-coumarate + H2O
-
 Pleurotus giganteus 4-coumarate + methanol
-
 ?
methyl caffeate + H2O
-
 Pleurotus giganteus caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Pleurotus giganteus ferulate + methanol
-
 ?
additional information the single purified enzyme brings about the release of ferulic acid from wheat bran and corn bran, and the amount released from wheat bran rises to 51.9% (of the total amount) by the synergistic action of xylanase Pleurotus giganteus ?
-
 ?
additional information no activity with 4-nitrophenyl palmitate and 4-nitrophenyl stearate Pleurotus giganteus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Pleurotus giganteus

Synonyms

Synonyms Comment Organism
FAE
-
 Pleurotus giganteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pleurotus giganteus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 68% of maximal activity at 20°C, 45% at 90°C, inactivation at 100°C Pleurotus giganteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 inactivation Pleurotus giganteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Pleurotus giganteus

pH Range

pH Minimum pH Maximum Comment Organism
2 7 60% of maximal activity at pH 2.0, maximal activity at pH 4.0, very low activity at pH 7.0, inactive at pH 8.0 Pleurotus giganteus