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Literature summary for 3.1.1.73 extracted from
- Metagenomic mining of feruloyl esterases from termite enteric flora (2014), Appl. Microbiol. Biotechnol., 98, 727-737.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fae1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) | uncultured bacterium |
| gene fae2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) | uncultured bacterium |
| gene fae3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) | uncultured bacterium |
| gene fae4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) | uncultured bacterium |
| gene fae5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) | uncultured bacterium |
| gene fae6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) | uncultured bacterium |
| gene fae7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, heterologous expression in Escherichia coli strain BL21(DE3) | uncultured bacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | - |
isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae5 | - |
| uncultured bacterium | M9VR53 | FAE4; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae4 | - |
| uncultured bacterium | M9VTY8 | FAE3; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae3 | - |
| uncultured bacterium | M9VUD6 | FAE2; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae2 | - |
| uncultured bacterium | M9VUE0 | FAE7; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae7 | - |
| uncultured bacterium | M9VY17 | FAE1; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae1 | - |
| uncultured bacterium | M9VY20 | FAE6; isolated from midgut of Trinervitermes trinervoides, collected from Komatipoort, Mpumalanga, South Africa, gene fae6 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl ferulate + H2O | - |
uncultured bacterium | ferulate + ethanol | - |
? |  |
| methyl 4-coumarate + H2O | low activity | uncultured bacterium | 4-coumarate + methanol | - |
? | |
| methyl caffeate + H2O | low activity | uncultured bacterium | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
uncultured bacterium | ferulate + methanol | - |
? | |
| methyl sinapinate + H2O | preferred substrate | uncultured bacterium | sinapate + methanol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAE1 | - |
uncultured bacterium |
| FAE2 | - |
uncultured bacterium |
| FAE3 | - |
uncultured bacterium |
| FAE4 | - |
uncultured bacterium |
| FAE5 | - |
uncultured bacterium |
| FAE6 | - |
uncultured bacterium |
| FAE7 | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
uncultured bacterium |
| 50 | - |
- |
uncultured bacterium |
| 60 | - |
- |
uncultured bacterium |
| 70 | - |
- |
uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | activity range | uncultured bacterium |
| 30 | 60 | activity range | uncultured bacterium |
| 40 | 60 | activity range | uncultured bacterium |
| 50 | 90 | activity range | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme, half-life is over 8 h | uncultured bacterium |
| 30 | 50 | recombinant enzyme, half-life is over 8 h | uncultured bacterium |
| 30 | 70 | recombinant enzyme, half-life is over 8 h | uncultured bacterium |
| 30 | 40 | recombinant enzyme, half-life is over 8 h | uncultured bacterium |
| 30 | 60 | recombinant enzyme, half-life is over 8 h | uncultured bacterium |
| 40 | - |
recombinant enzyme, half-life is 7 h | uncultured bacterium |
| 50 | - |
recombinant enzyme, half-life is 1.5 h | uncultured bacterium |
| 50 | - |
recombinant enzyme, half-life is below 1 h | uncultured bacterium |
| 60 | - |
recombinant enzyme, half-life is 6 h | uncultured bacterium |
| 60 | - |
recombinant enzyme, inactivation | uncultured bacterium |
| 70 | - |
recombinant enzyme, half-life is below 1 h | uncultured bacterium |
| 70 | - |
recombinant enzyme, inactivation | uncultured bacterium |
| 80 | - |
recombinant enzyme, half-life is 2.5 h | uncultured bacterium |
| 80 | - |
recombinant enzyme, inactivation | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | over 60% of maximal activity within this range | uncultured bacterium |
| 6 | 7 | over 60% of maximal activity within this range | uncultured bacterium |
| 7 | 8 | over 60% of maximal activity within this range | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the termite-derived isozymes form a distinct subclass of feroyl esterases | uncultured bacterium |
| additional information | the catalytic triad is formed by Ser121, Asp215 and His244 | uncultured bacterium |
| additional information | the catalytic triad is formed by Ser123, Asp212 and His241 | uncultured bacterium |
| additional information | the catalytic triad is formed by Ser124, Asp215 and His244 | uncultured bacterium |
| additional information | the catalytic triad is formed by Ser125, Asp216 and His245 | uncultured bacterium |
| additional information | the catalytic triad is formed by Ser126, Asp215 and His244 | uncultured bacterium |
| additional information | the catalytic triad is formed by Ser128, Asp215 and His244 | uncultured bacterium |
| additional information | the catalytic triad is formed by Ser131, Asp222 and His251 | uncultured bacterium |
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