Literature summary for 3.1.1.73 extracted from

Chen, S.K.; Wang, K.; Liu, Y.; Hu, X.
Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library (2012), Acta Crystallogr. Sect. F, 68, 767-770.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, hanging drop vapour diffusion, 0.002 ml of 15 mg/ml protein in 200 mM NaCl, 10 mM HEPES, pH 7.5, 5 mM dithiothreitol, 10% glycerol, are mixed with 0.002 ml of reservoir solution containing 0.2 M ammonium sulfate, 26% PEG 4000, 0.1 M sodium cacodylate pH 6.5, 4% w/v benzamidine-HCl, and equilibration against 0.5 ml reservoir solution, 16°C, 2 weeks, method optimization, X-ray diffraction structure determination and analysis at 2.9 A resolution	uncultured bacterium

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme, hanging drop vapour diffusion, 0.002 ml of 15 mg/ml protein in 200 mM NaCl, 10 mM HEPES, pH 7.5, 5 mM dithiothreitol, 10% glycerol, are mixed with 0.002 ml of reservoir solution containing 0.2 M ammonium sulfate, 26% PEG 4000, 0.1 M sodium cacodylate pH 6.5, 4% w/v benzamidine-HCl, and equilibration against 0.5 ml reservoir solution, 16°C, 2 weeks, method optimization, X-ray diffraction structure determination and analysis at 2.9 A resolution

uncultured bacterium

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	E7DJY5	gene EstF27 from a soil metagenomic library from agricultural soil	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzymefrom Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration	uncultured bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
21.7	-	substrate 4-nitrophenyl ferulate, pH and temperature not specified in the publication	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl ferulate + H2O	-	uncultured bacterium	4-nitrophenol + ferulate	-	?

Synonyms

Synonyms	Comment	Organism
EstF27	-	uncultured bacterium
FAE	-	uncultured bacterium
ferulic acid esterase	-	uncultured bacterium