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Literature summary for 3.1.1.73 extracted from
- Novel bacterial ferulic acid esterase from Cellvibrio japonicus and its application in ferulic acid release and xylan hydrolysis (2011), Biotechnol. Lett., 33, 47-54.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes fee1A and fee1B, DNA and mino acid sequence determination and analysis, subcloning in Escherichia coli strain JM109, expression as His-tagged Fee1B in Escherichia coli strain BL21(DE3) | Cellvibrio japonicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a truncated Feel1B variant with reduced enzyme activity | Cellvibrio japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellvibrio japonicus | - |
genes fee1A and fee1B | - |
| Cellvibrio japonicus NCIMB 10462 | - |
genes fee1A and fee1B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Fee1B 12fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Cellvibrio japonicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
above, purified recombinant enzyme, substrate is methyl caffeate, pH 7.0, 40°C | Cellvibrio japonicus |
| 10.5 | - |
above, purified recombinant enzyme, substrate is methyl sinapate, pH 7.0, 40°C | Cellvibrio japonicus |
| 12.4 | - |
above, purified recombinant enzyme, substrate is methyl 4-coumarate, pH 7.0, 40°C | Cellvibrio japonicus |
| 28 | - |
above, purified recombinant enzyme, substrate is methyl ferulate, pH 7.0, 40°C | Cellvibrio japonicus |
| 50 | - |
purifed recombinant enzyme, substrate is 4-nitrophenyl acetate, pH 7.0, 40°C | Cellvibrio japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Cellvibrio japonicus | 4-nitrophenol + acetate | - |
? |  |
| 4-nitrophenyl acetate + H2O | - |
Cellvibrio japonicus NCIMB 10462 | 4-nitrophenol + acetate | - |
? | |
| methyl 4-coumarate + H2O | - |
Cellvibrio japonicus | 4-coumaric acid + methanol | - |
? | |
| methyl caffeate + H2O | - |
Cellvibrio japonicus | caffeic acid + methanol | - |
? | |
| methyl caffeate + H2O | - |
Cellvibrio japonicus NCIMB 10462 | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Cellvibrio japonicus | ferulic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Cellvibrio japonicus NCIMB 10462 | ferulic acid + methanol | - |
? | |
| methyl sinapate + H2O | - |
Cellvibrio japonicus | sinapic acid + methanol | - |
? | |
| methyl sinapate + H2O | - |
Cellvibrio japonicus NCIMB 10462 | sinapic acid + methanol | - |
? | |
| additional information | substrate specificity of Fee1B, overview | Cellvibrio japonicus | ? | - |
? | |
| additional information | substrate specificity of Fee1B, overview | Cellvibrio japonicus NCIMB 10462 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAE | - |
Cellvibrio japonicus |
| Fee1B | - |
Cellvibrio japonicus |
| ferulic acid esterase | - |
Cellvibrio japonicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Cellvibrio japonicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | activity range of Fee1B, profile overview | Cellvibrio japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
purified recombinant Fee1B is stable at temperature up to 40°C. The enzyme loses about 30% activity upon incubation at 45°C for 30 min | Cellvibrio japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Cellvibrio japonicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10 | activity range of Fee1B, profile overview | Cellvibrio japonicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified recombinant Fee1B is fairly stable over a wide range of pH | Cellvibrio japonicus |
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Release 2023.1 (January 2023)
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