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Literature summary for 3.1.1.73 extracted from

  • Zhang, S.B.; Wu, Z.L.
    Identification of amino acid residues responsible for increased thermostability of feruloyl esterase A from Aspergillus niger using the PoPMuSiC algorithm (2011), Biores. Technol., 102, 2093-2096.
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Pichia pastoris strain KM71 Aspergillus niger

Protein Variants

Protein Variants Comment Organism
D174A site-directed mutagenesis, the mutant does not show changes in thermal stability compared to the wild-type enzyme Aspergillus niger
D93G site-directed mutagenesis, the mutant shows slightly increased thermal stability compared to the wild-type enzyme Aspergillus niger
S187F site-directed mutagenesis, the mutant shows highly increased thermal stability compared to the wild-type enzyme. The substitution of S187F reduces three possible hydrogen bonds Aspergillus niger
S92A site-directed mutagenesis, the mutant does not show changes in thermal stability compared to the wild-type enzyme Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics, overview Aspergillus niger
12.87
-
 ferulate pH 6.4, 40°C, mutant D93G Aspergillus niger
14.4
-
 ferulate pH 6.4, 40°C, wild-type enzyme Aspergillus niger
20.1
-
 ferulate pH 6.4, 40°C, mutant S187F Aspergillus niger
23.35
-
 ferulate pH 6.4, 40°C, mutant D93G/S187F Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger O42807
-
-
Aspergillus niger CIB 423.1 O42807
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.5
-
 wild-type enzyme, substrate 4-nitrophenyl ferulate, pH 6.4, 40°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl ferulate + H2O
-
 Aspergillus niger 4-nitrophenol + ferulate
-
 ?
4-nitrophenyl ferulate + H2O
-
 Aspergillus niger CIB 423.1 4-nitrophenol + ferulate
-
 ?
methyl ferulate + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Aspergillus niger CIB 423.1 ferulic acid + methanol
-
 ?

Synonyms

Synonyms Comment Organism
FAEA
-
 Aspergillus niger
feruloyl esterase A
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-lives of wild-type enzyme and mutants S92A and D174A is 8 min, half-life of mutant D93G is 9.4 min, and of mutant S187F 60.5 min Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
62
-
 ferulate pH 6.4, 40°C, wild-type enzyme Aspergillus niger
84
-
 ferulate pH 6.4, 40°C, mutant D93G Aspergillus niger
790
-
 ferulate pH 6.4, 40°C, mutant D93G/S187F Aspergillus niger
823
-
 ferulate pH 6.4, 40°C, mutant S187F Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 assay at Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3
-
 ferulate pH 6.4, 40°C, wild-type enzyme Aspergillus niger
6.5
-
 ferulate pH 6.4, 40°C, mutant D93G Aspergillus niger
34
-
 ferulate pH 6.4, 40°C, mutant D93G/S187F Aspergillus niger
41
-
 ferulate pH 6.4, 40°C, mutant S187F Aspergillus niger