Application | Comment | Organism |
---|---|---|
industry | bioproduction of ferulic acid from triticale bran by alkaline and thermostable FAE | Caldanaerobacter subterraneus subsp. tengcongensis |
Cloned (Comment) | Organism |
---|---|
777-bp DNA fragment digested with EcoRI and PstI and ligated into the corresponding sites of the IPTG-inducible plasmid pKK223-3, transformed into Escherichia coli strain JM109 and expressed from pKTtFAE vector | Caldanaerobacter subterraneus subsp. tengcongensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
methyl p-coumarate | at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis | |
0.29 | - |
methyl sinapate | at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis | |
0.32 | - |
methyl ferulate | at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29100 | - |
sequence analysis | Caldanaerobacter subterraneus subsp. tengcongensis |
33000 | - |
1 * 33000, SDS-PAGE, purified enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
36000 | - |
native enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
- |
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity by centrifugation, ammonium sulfate precipitation, ultrafiltration and gel filtration, 30.5fold with a yield of 9% | Caldanaerobacter subterraneus subsp. tengcongensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
with 5 mM methyl syringate as substrate only traces, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
0.0004 | - |
with 1 mM p-nitrophenyl palmitate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
0.03 | - |
with 5 mM methyl vanillate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
0.05 | - |
with 5 mM methyl caffeate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
0.426 | - |
with 5 mM methyl p-coumarate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
0.456 | - |
with 5 mM methyl ferulate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
0.462 | - |
with feruloylated oligosaccharides as substrate, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
0.511 | - |
with 5 mM methyl sinapate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
1.7 | - |
with 1 mM p-nitrophenyl myristate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
10.5 | - |
with 1 mM p-nitrophenyl caprylate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
18.5 | - |
with 1 mM p-nitrophenyl acetate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
36 | - |
with 1 mM p-nitrophenyl butyrate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloylated oligosaccharide + H2O | ferulic acid is released from feruloylated oligosaccharides prepared from triticale bran. Recovery of 68 mg ferulic acid/100 g triticale bran by a 30% release of the total ferulic acid from triticale bran within a 5 h incubation period. Trichoderma viride xylanase combined with FAE can release 29.2 mg/100 g material | Caldanaerobacter subterraneus subsp. tengcongensis | ferulic acid + oligosaccharide | - |
? | |
methyl caffeate + H2O | poor substrate | Caldanaerobacter subterraneus subsp. tengcongensis | caffeic acid + methanol | - |
? | |
methyl caffeate + H2O | poor substrate | Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 | caffeic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | ferulic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 | ferulic acid + methanol | - |
? | |
methyl p-coumarate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | p-coumaric acid + methanol | - |
? | |
methyl sinapate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | sinapic acid + methanol | - |
? | |
methyl syringate + H2O | poor substrate | Caldanaerobacter subterraneus subsp. tengcongensis | ? + methanol | - |
? | |
methyl vanillate + H2O | poor substrate | Caldanaerobacter subterraneus subsp. tengcongensis | ? + methanol | - |
? | |
p-nitrophenyl acetate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | p-nitrophenol + acetic acid | - |
? | |
p-nitrophenyl acetate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 | p-nitrophenol + acetic acid | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | p-nitrophenol + butyric acid | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 | p-nitrophenol + butyric acid | - |
? | |
p-nitrophenyl caprylate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | p-nitrophenol + caprylate | - |
? | |
p-nitrophenyl myristate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | p-nitrophenol + myristate | - |
? | |
p-nitrophenyl palmitate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis | p-nitrophenol + palmitate | - |
? | |
p-nitrophenyl palmitate + H2O | - |
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 | p-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE, purified enzyme | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms | Comment | Organism |
---|---|---|
FAE | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
feruloyl esterase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | at 75°C retains at least 95% of its original activity for over 80 min. At 80°C, its half-life is 50 min, rendering FAE a highly thermostable protein | Caldanaerobacter subterraneus subsp. tengcongensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
methyl p-coumarate | at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis | |
0.28 | - |
methyl ferulate | at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis | |
0.28 | - |
methyl sinapate | at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 | Caldanaerobacter subterraneus subsp. tengcongensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
purified enzyme, in 50 mM Britton and Robinson's universal buffer | Caldanaerobacter subterraneus subsp. tengcongensis |