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Literature summary for 3.1.1.73 extracted from

  • Benoit, I.; Asther, M.; Sulzenbacher, G.; Record, E.; Marmuse, L.; Parsiegla, G.; Gimbert, I.; Asther, M.; Bignon, C.
    Respective importance of protein folding and glycosylation in the thermal stability of recombinant feruloyl esterase A (2006), FEBS Lett., 580, 5815-5821.
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus niger

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, FAEA produced in Aspergillus niger (AnN) and Escherichia coli (EcR) Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
diethyl-2-(4-hydroxy-3-methoxyphenyl)ethenephosphonate
-
 Aspergillus niger
methyl 5-N-arabinoferulamide
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 methyl ferulate FAEA produced in Escherichia coli (EcR) Aspergillus niger
0.15
-
 methyl sinapate FAEA produced in Escherichia coli (EcR) Aspergillus niger
0.31
-
 methyl ferulate FAEA produced in Aspergillus niger Aspergillus niger
0.37
-
 methyl sinapate FAEA produced in Aspergillus niger Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28550
-
 FAEA produced in Escherichia coli, mass spectrometry Aspergillus niger
30250
-
 FAEA produced in Aspergillus niger, mass spectrometry Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger O42807
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-sc l-arabinofuranoside + H2O
-
 Aspergillus niger ?
-
 ?
methyl ferulate + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
methyl sinapate + H2O
-
 Aspergillus niger sinapic acid + methanol
-
 ?

Synonyms

Synonyms Comment Organism
FAEA
-
 Aspergillus niger
feruloyl esterase A
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 substrate: methyl ferulate, FAEA produced in Escherichia coli (EcR) Aspergillus niger
55
-
 substrate: methyl ferulate, FAEA produced in Aspergillus niger (AnN) Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 30 min, FAEA produced in Aspergillus niger loses 15% of its activity, FAEA produced in Escherichia coli loses 94% of its activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 substrate: methyl ferulate, FAEA produced in Aspergillus niger (AnN) and Escherichia coli (EcR) Aspergillus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4
-
 methyl 5-N-arabinoferulamide substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Escherichia coli (EcR) Aspergillus niger
1.45
-
 diethyl-2-(4-hydroxy-3-methoxyphenyl)ethenephosphonate substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Aspergillus niger Aspergillus niger
2.5
-
 diethyl-2-(4-hydroxy-3-methoxyphenyl)ethenephosphonate substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Aspergillus niger Aspergillus niger
2.9
-
 methyl 5-N-arabinoferulamide substrate: 2-chloro 4-nitrophenyl 5-O-feruloyl-alpha-L-arabinofuranoside, FAEA produced in Escherichia coli (EcR) Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger calculation from sequence and verification by isoelectric focusing, FAEA produced in Aspergillus niger (AnN)
-
 4.2