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Literature summary for 3.1.1.73 extracted from

  • Levasseur, A.; Benoit, I.; Asther, M.; Asther, M.; Record, E.
    Homologous expression of the feruloyl esterase B gene from Aspergillus niger and characterization of the recombinant enzyme (2004), Protein Expr. Purif., 37, 126-133.
    View publication on PubMed
Search for more literature for 3.1.1.73

Application

Application Comment Organism
nutrition broad range of application in the agri-food industries, production of ferulic as food preservative and as potential precursor for the production of flavor and photo-active agents Aspergillus niger
paper production potential use in pulp bleaching Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in a protease deficient Aspergillus niger strain Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 methyl p-coumarate
-
 Aspergillus niger
0.13
-
 methyl caffeate
-
 Aspergillus niger
0.16
-
 5-O-caffeoyl quinic acid
-
 Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 2 * 75000, SDS-PAGE, native mass by gel filtration Aspergillus niger
150000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Aspergillus niger involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O Aspergillus niger BRFM131 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger Q8WZI8 feruloyl esterase B
-
Aspergillus niger BRFM131 Q8WZI8 feruloyl esterase B
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-O-caffeoyl quinic acid + H2O
-
 Aspergillus niger ?
-
 ?
5-O-caffeoyl quinic acid + H2O
-
 Aspergillus niger BRFM131 ?
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus niger ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus niger BRFM131 ferulic acid + ?
-
 ?
methyl caffeate + H2O
-
 Aspergillus niger caffeic acid + methanol
-
 ?
methyl caffeate + H2O
-
 Aspergillus niger BRFM131 caffeic acid + methanol
-
 ?
methyl p-coumarate + H2O
-
 Aspergillus niger p-coumaric acid + methanol
-
 ?
methyl p-coumarate + H2O
-
 Aspergillus niger BRFM131 p-coumaric acid + methanol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 75000, SDS-PAGE, native mass by gel filtration Aspergillus niger

Synonyms

Synonyms Comment Organism
FAEB
-
 Aspergillus niger
feruloyl esterase B
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable Aspergillus niger
45 50 10-16% loss of activity within 120 min Aspergillus niger
55
-
 half life: 30 min Aspergillus niger
60
-
 rapid inactivation Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger isoelectric focusing
-
 4.8