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Literature summary for 3.1.1.73 extracted from
- Production and characterization of the Talaromyces stipitatus feruloyl esterase FAEC in Pichia pastoris: identification of the nucleophilic serine (2003), Protein Expr. Purif., 29, 176-184.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | broad range of application in the agri-food industries, production of ferulic as food preservative and as potential precursor for the production of flavor and photo-active agents | Talaromyces stipitatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Talaromyces stipitatus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | additional amino acids at the N-terminus do not influence the activity | Talaromyces stipitatus |
| S166A | complete loss of activity | Talaromyces stipitatus |
| S465A | no effect on activity | Talaromyces stipitatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
methyl p-coumarate | pH 6.0, 37°C | Talaromyces stipitatus |  |
| 0.04 | - |
methyl ferulate | pH 6.0, 37°C | Talaromyces stipitatus | |
| 0.37 | - |
methyl sinapinate | pH 6.0, 37°C | Talaromyces stipitatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Talaromyces stipitatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55340 | - |
1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE | Talaromyces stipitatus |
| 72000 | - |
1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE | Talaromyces stipitatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | Talaromyces stipitatus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
| feruloyl polysaccharide + H2O | Talaromyces stipitatus CBS 375.48 | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
| hemicellulose + H2O | Talaromyces stipitatus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + lignin + ? | - |
? | |
| hemicellulose + H2O | Talaromyces stipitatus CBS 375.48 | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + lignin + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces stipitatus | Q70Y21 | - |
- |
| Talaromyces stipitatus CBS 375.48 | Q70Y21 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Talaromyces stipitatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Talaromyces stipitatus | ferulic acid + ? | - |
? | |
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Talaromyces stipitatus CBS 375.48 | ferulic acid + ? | - |
? | |
| hemicellulose + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Talaromyces stipitatus | ferulic acid + lignin + ? | - |
? | |
| hemicellulose + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Talaromyces stipitatus CBS 375.48 | ferulic acid + lignin + ? | - |
? | |
| methyl caffeate + H2O | - |
Talaromyces stipitatus | caffeic acid + methanol | - |
? | |
| methyl caffeate + H2O | - |
Talaromyces stipitatus CBS 375.48 | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Talaromyces stipitatus | ferulic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Talaromyces stipitatus CBS 375.48 | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + H2O | - |
Talaromyces stipitatus | p-coumaric acid + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Talaromyces stipitatus | sinapic acid + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Talaromyces stipitatus CBS 375.48 | sinapic acid + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55340, calculated from the deduced amino acid sequence, 1 * 72000, SDS-PAGE | Talaromyces stipitatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | 1.97 | methyl sinapinate | pH 6.0, 37°C | Talaromyces stipitatus | |
| 9.26 | - |
methyl sinapinate | pH 6.0, 37°C | Talaromyces stipitatus | |
| 9.55 | - |
methyl p-coumarate | pH 6.0, 37°C | Talaromyces stipitatus | |
| 9.65 | - |
methyl ferulate | pH 6.0, 37°C | Talaromyces stipitatus | |
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