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Literature summary for 3.1.1.73 extracted from

  • Topakas, E.; Vafiadi, C.; Stamatis, H.; Christakopoulos, P.
    Sporotrichum thermophile type C feruloyl esterase (StFaeC): purification, characterization, and its use for phenolic acid (sugar) ester synthesis (2005), Enzyme Microb. Technol., 36, 729-736.
No PubMed abstract available
Search for more literature for 3.1.1.73

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 methyl caffeate
-
 Thermothelomyces heterothallicus
0.57
-
 methyl sinapinate
-
 Thermothelomyces heterothallicus
0.57
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside
-
 Thermothelomyces heterothallicus
0.59
-
 methyl p-coumarate
-
 Thermothelomyces heterothallicus
0.62
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside
-
 Thermothelomyces heterothallicus
1.64
-
 methyl ferulate
-
 Thermothelomyces heterothallicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermothelomyces heterothallicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 23000, SDS-PAGE, native mass by native PAGE Thermothelomyces heterothallicus
43000
-
 native PAGE Thermothelomyces heterothallicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Thermothelomyces heterothallicus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
wheat bran + H2O Thermothelomyces heterothallicus
-
 ferulic acid + ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information activity is retained in hexane/1-butanol/water surfactantless microemulsion Thermothelomyces heterothallicus

Organism

Organism UniProt Comment Textmining
Thermothelomyces heterothallicus
-
 strain ATCC 34628, feruloyl esterase type C
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermothelomyces heterothallicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
 purified enzyme Thermothelomyces heterothallicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate Thermothelomyces heterothallicus ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O C-5 linked ferulate is hydrolyzed three times more efficiently than C-2 linked ferulate Thermothelomyces heterothallicus ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Thermothelomyces heterothallicus ferulic acid + ?
-
 ?
methyl caffeate + H2O
-
 Thermothelomyces heterothallicus caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Thermothelomyces heterothallicus ferulic acid + methanol
-
 ?
methyl p-coumarate + H2O
-
 Thermothelomyces heterothallicus p-coumaric acid + methanol
-
 ?
methyl sinapinate + H2O
-
 Thermothelomyces heterothallicus sinapic acid + methanol
-
 ?
wheat bran + H2O
-
 Thermothelomyces heterothallicus ferulic acid + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE, native mass by native PAGE Thermothelomyces heterothallicus

Synonyms

Synonyms Comment Organism
StFaeC
-
 Thermothelomyces heterothallicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermothelomyces heterothallicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 82% of activity Thermothelomyces heterothallicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 methyl caffeate
-
 Thermothelomyces heterothallicus
0.4
-
 methyl sinapinate
-
 Thermothelomyces heterothallicus
0.5
-
 methyl p-coumarate
-
 Thermothelomyces heterothallicus
2.6
-
 methyl ferulate
-
 Thermothelomyces heterothallicus
3.7
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside
-
 Thermothelomyces heterothallicus
10.3
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside
-
 Thermothelomyces heterothallicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Thermothelomyces heterothallicus

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 80% of activity Thermothelomyces heterothallicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 stable, 80% of activity after 24 h at pH 10 Thermothelomyces heterothallicus
10
-
 half life at 55°C: 133 min, half life at 60°C: 55 min Thermothelomyces heterothallicus

pI Value

Organism Comment pI Value Maximum pI Value
Thermothelomyces heterothallicus isoelectric focusing and cation-exchange chromatofocusing
-
 3.1