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Literature summary for 3.1.1.73 extracted from
- Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application (2003), Appl. Microbiol. Biotechnol., 62, 349-355.
Application
| Application | Comment | Organism |
|---|---|---|
| paper production | potential use in pulp bleaching, application of recombinant FAEA in combination with laccase and xylanase for efficient delignification of wheat straw pulp | Aspergillus niger |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Aspergillus niger strain D15-26 (pyrg-) | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
methyl ferulate | - |
Aspergillus niger |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | Aspergillus niger | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
| hemicellulose + H2O | Aspergillus niger | hydrolysis of feruloylated oligosaccharides and diferulate cross-links | ferulic acid + lignin + pectin + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
glucose as carbon source yields a higher activity than maltose | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Aspergillus niger | ferulic acid + ? | - |
? | |
| hemicellulose + H2O | hydrolysis of feruloylated oligosaccharides and diferulate cross-links | Aspergillus niger | ferulic acid + lignin + pectin + ? | - |
? | |
| methyl ferulate + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Aspergillus niger | sinapic acid + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectric focusing | - |
3.3 |
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