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Literature summary for 3.1.1.73 extracted from

  • Biely, P.; Mastihubova, M.; van Zyl, W.H.; Prior, B.A.
    Differentiation of feruloyl esterases on synthetic substrates in alpha-arabinofuranosidase-coupled and ultraviolet-spectrophotometric assays (2002), Anal. Biochem., 311, 68-75.
    View publication on PubMed
Search for more literature for 3.1.1.73

Application

Application Comment Organism
synthesis isolation of phenolic acids from lignocellulosic wastes as precursors for the synthesis of chemicals Aspergillus oryzae
synthesis potential application in the bioconversion of lignocellulosic wastes yielding ferulic acid as precursor for chemical synthesis Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside pH 5.5, 30°C Aspergillus oryzae
0.28
-
 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside pH 5.5, 30°C Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus oryzae
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Aspergillus oryzae involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
hemicellulose + H2O Aspergillus oryzae involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + arabinoxylan + pectin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O O-5 linkage is hydrolyzed much faster than the O-2 linkage Aspergillus oryzae ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O suitable substrate for the determination of enzyme activity in a coupled assay together with alpha-L-arabinofuranosidase Aspergillus oryzae ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O O-5 linkage is hydrolyzed much faster than the O-2 linkage Aspergillus oryzae ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O suitable substrate for the determination of enzyme activity in a coupled assay together with alpha-L-arabinofuranosidase Aspergillus oryzae ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus oryzae ferulic acid + ?
-
 ?
hemicellulose + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus oryzae ferulic acid + arabinoxylan + pectin + ?
-
 ?