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Literature summary for 3.1.1.73 extracted from

  • Donaghy, J.A.; Bronnenmeier, K.; Soto-Kelly, P.F.; McKay, A.M.
    Purification and characterization of an extracellular feruloyl esterase from the thermophilic anaerobe Clostridium stercorarium (2000), J. Appl. Microbiol., 88, 458-466.
    View publication on PubMed

Application

Application Comment Organism
nutrition
-
Aspergillus awamori
nutrition
-
Penicillium expansum
nutrition enzymes capable of cleaving phenolic acid-ester cross-linkages have applications in food and food-related industries like baking industry, food flavour industry, animal nutrition and plant processing Thermoclostridium stercorarium

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Thermoclostridium stercorarium
Co2+
-
Thermoclostridium stercorarium
Cu2+
-
Penicillium expansum
Cu2+
-
Talaromyces pinophilus
Cu2+
-
Thermoclostridium stercorarium
Fe3+
-
Thermoclostridium stercorarium
Mg2+
-
Thermoclostridium stercorarium
Mn2+
-
Thermoclostridium stercorarium
Zn2+
-
Aspergillus awamori
Zn2+
-
Thermoclostridium stercorarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
methyl ferulate
-
Thermoclostridium stercorarium
0.86
-
methyl p-coumarate
-
Thermoclostridium stercorarium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces olivochromogenes
-
-
extracellular
-
Aspergillus sp.
-
-
extracellular
-
Aspergillus awamori
-
-
extracellular
-
Thermoclostridium stercorarium
-
-
extracellular
-
Penicillium expansum
-
-
extracellular
-
Talaromyces pinophilus
-
-
extracellular
-
Neocallimastix sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
gel filtration, anomalous retardation of the purified enzyme Thermoclostridium stercorarium
33000
-
SDS-PAGE, gel filtration Thermoclostridium stercorarium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
birchwood xylan + H2O Thermoclostridium stercorarium
-
ferulic acid + ?
-
?
feruloyl-polysaccharide + H2O Pseudomonas fluorescens
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Streptomyces olivochromogenes
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Fibrobacter succinogenes
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Aspergillus sp.
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Schizophyllum commune
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Aspergillus awamori
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Thermoclostridium stercorarium
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Penicillium expansum
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Streptomyces viridosporus
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Talaromyces pinophilus
-
ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O Neocallimastix sp.
-
ferulate + polysaccharide
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus sp.
-
-
-
Fibrobacter succinogenes
-
-
-
Neocallimastix sp.
-
-
-
Penicillium expansum
-
-
-
Pseudomonas fluorescens
-
subsp. cellulosa
-
Schizophyllum commune
-
-
-
Streptomyces olivochromogenes
-
-
-
Streptomyces viridosporus
-
-
-
Talaromyces pinophilus
-
-
-
Thermoclostridium stercorarium
-
NCIMB 11754
-
Thermoclostridium stercorarium NCIMB 11754
-
NCIMB 11754
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoclostridium stercorarium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
methyl caffeate as substrate Thermoclostridium stercorarium
58
-
methyl coumarate as substrate Thermoclostridium stercorarium
63
-
methyl sinapate as substrate Thermoclostridium stercorarium
75
-
methyl ferulate as substrate Thermoclostridium stercorarium
88
-
ethyl ferulate as substrate Thermoclostridium stercorarium

Storage Stability

Storage Stability Organism
-20°C, approximately 70% original activity retained after storage for 3 months Thermoclostridium stercorarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
Thermoclostridium stercorarium ferulic acid + ?
-
?
de-starched wheat bran + H2O
-
Streptomyces olivochromogenes ferulic acid + ?
-
?
de-starched wheat bran + H2O
-
Schizophyllum commune ferulic acid + ?
-
?
de-starched wheat bran + H2O
-
Thermoclostridium stercorarium ferulic acid + ?
-
?
de-starched wheat bran + H2O
-
Talaromyces pinophilus ferulic acid + ?
-
?
de-starched wheat bran + H2O
-
Thermoclostridium stercorarium NCIMB 11754 ferulic acid + ?
-
?
ethyl ferulate + H2O
-
Thermoclostridium stercorarium ferulic acid + ethanol
-
?
ethyl ferulate + H2O
-
Thermoclostridium stercorarium NCIMB 11754 ferulic acid + ethanol
-
?
ferulic acid methyl ester + H2O
-
Streptomyces olivochromogenes ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Aspergillus sp. ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Aspergillus awamori ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Thermoclostridium stercorarium ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Penicillium expansum ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Talaromyces pinophilus ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Neocallimastix sp. ferulic acid + methanol
-
?
ferulic acid methyl ester + H2O
-
Thermoclostridium stercorarium NCIMB 11754 ferulic acid + methanol
-
?
feruloyl-polysaccharide + H2O
-
Pseudomonas fluorescens ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Streptomyces olivochromogenes ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Fibrobacter succinogenes ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus sp. ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Schizophyllum commune ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Aspergillus awamori ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Thermoclostridium stercorarium ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Penicillium expansum ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Streptomyces viridosporus ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Talaromyces pinophilus ferulate + polysaccharide
-
?
feruloyl-polysaccharide + H2O
-
Neocallimastix sp. ferulate + polysaccharide
-
?
methyl caffeate + H2O
-
Thermoclostridium stercorarium caffeic acid + methanol
-
?
methyl caffeate + H2O
-
Penicillium expansum caffeic acid + methanol
-
?
methyl sinapinate + H2O
-
Streptomyces olivochromogenes sinapic acid + methanol
-
?
methyl sinapinate + H2O
-
Thermoclostridium stercorarium sinapic acid + methanol
-
?
additional information
-
Pseudomonas fluorescens ?
-
?
additional information
-
Streptomyces olivochromogenes ?
-
?
additional information
-
Aspergillus sp. ?
-
?
additional information
-
Aspergillus awamori ?
-
?
additional information
-
Penicillium expansum ?
-
?
additional information
-
Talaromyces pinophilus ?
-
?
additional information
-
Neocallimastix sp. ?
-
?
additional information no activity towards the benzoic acid esters methyl syringate and methyl vanillate Thermoclostridium stercorarium ?
-
?
additional information no activity towards the benzoic acid esters methyl syringate and methyl vanillate Thermoclostridium stercorarium NCIMB 11754 ?
-
?
O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Aspergillus awamori ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
?
O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Thermoclostridium stercorarium ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
?
O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Penicillium expansum ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
?
O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O FAXX Neocallimastix sp. ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
?
p-coumaric acid methyl ester + H2O
-
Streptomyces olivochromogenes p-coumaric acid + methanol
-
?
p-coumaric acid methyl ester + H2O
-
Aspergillus sp. p-coumaric acid + methanol
-
?
p-coumaric acid methyl ester + H2O
-
Aspergillus awamori p-coumaric acid + methanol
-
?
p-coumaric acid methyl ester + H2O
-
Thermoclostridium stercorarium p-coumaric acid + methanol
-
?
p-coumaric acid methyl ester + H2O
-
Talaromyces pinophilus p-coumaric acid + methanol
-
?
p-coumaric acid methyl ester + H2O
-
Thermoclostridium stercorarium NCIMB 11754 p-coumaric acid + methanol
-
?

Subunits

Subunits Comment Organism
monomer
-
Streptomyces olivochromogenes
monomer
-
Aspergillus awamori
monomer
-
Talaromyces pinophilus
monomer
-
Neocallimastix sp.
monomer 1 * 33000, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermoclostridium stercorarium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
activity rapidly decreases above, loses 30% activity after 15 min Thermoclostridium stercorarium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thermoclostridium stercorarium
9
-
-
Streptomyces viridosporus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
Thermoclostridium stercorarium