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Literature summary for 3.1.1.73 extracted from
- Purification and characterization of a feruloylesterase from Aspergillus awamori (1998), Biosci. Biotechnol. Biochem., 62, 2032-2034.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | a common fungus used in the fermentation of shochu | Aspergillus awamori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-ethyl-5-phenylisoxazolium-3'-sulfonate | - |
Aspergillus awamori |  |
| diisopropylfluorophosphate | - |
Aspergillus awamori | |
| p-bromophenacyl bromide | - |
Aspergillus awamori | |
| phenylmethylsulfonyl fluoride | - |
Aspergillus awamori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
1-acetoxynaphthalene | - |
Aspergillus awamori | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces olivochromogenes | - |
- |
| extracellular | - |
Aspergillus awamori | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Aspergillus awamori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus oryzae | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus awamori | - |
ferulate + polysaccharide | - |
? | |
| wheat bran + H2O | Aspergillus niger | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat bran + H2O | Aspergillus awamori | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Streptomyces olivochromogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
- |
Streptomyces olivochromogenes |
- |
Aspergillus oryzae |
- |
Aspergillus awamori |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 65.1 | - |
- |
Aspergillus awamori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Aspergillus awamori | acetic acid + 1-naphthol | - |
? | |
| 1-naphthyl butyrate + H2O | - |
Aspergillus awamori | butyric acid + 1-naphthol | - |
? | |
| 1-naphthyl pentanoate + H2O | - |
Aspergillus awamori | pentanoic acid + 1-naphthol | - |
? | |
| 1-naphthyl propionate + H2O | - |
Aspergillus awamori | propionic acid + 1-naphthol | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus oryzae | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus awamori | ferulate + polysaccharide | - |
? | |
| additional information | - |
Aspergillus niger | ? | - |
? | |
| additional information | - |
Streptomyces olivochromogenes | ? | - |
? | |
| additional information | beta-naphythyl esters no substrates of the enzyme | Aspergillus awamori | ? | - |
? | |
| wheat bran + H2O | - |
Aspergillus niger | ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat bran + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Streptomyces olivochromogenes |
| monomer | 1 * 35000, SDS-PAGE | Aspergillus awamori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Aspergillus awamori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to | Aspergillus awamori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
| 5 | - |
- |
Aspergillus awamori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Aspergillus awamori |
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