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Literature summary for 3.1.1.73 extracted from
- Purification and partial characterization of two feruloyl esterases from the anaerobic fungus Neocallimastix strain MC-2 (1992), Appl. Environ. Microbiol., 58, 3762-3766.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0096 | - |
O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | FAE-II | Neocallimastix sp. |  |
| 0.0319 | - |
O-5-O-(E)-feruloyl-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | FAE-I | Neocallimastix sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces olivochromogenes | - |
- |
| extracellular | - |
Neocallimastix sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
FAE-II, denaturing and nondenaturing PAGE, gel filtration, high performance gel filtration | Neocallimastix sp. |
| 29000 | - |
- |
Streptomyces olivochromogenes |
| 68000 | - |
FAE-I, high-performance gel filtration | Neocallimastix sp. |
| 69000 | - |
FAE-I, denaturing and nondenaturing PAGE, gel filtration | Neocallimastix sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Schizophyllum commune | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Neocallimastix sp. | - |
2 extracellular feruloyl esterases, FAE-I and FAE-II | - |
| Neocallimastix sp. | - |
rumen fungus, MC-2 | - |
| Schizophyllum commune | - |
- |
- |
| Streptomyces olivochromogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces olivochromogenes |
| p-coumaroyl esterase, FAE-I, FAE-II | Neocallimastix sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.9 | - |
FAE-I | Neocallimastix sp. |
| 11.2 | - |
FAE-II | Neocallimastix sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAX3-I | Neocallimastix sp. | ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| de-starched wheat bran + H2O | - |
Aspergillus niger | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Streptomyces olivochromogenes | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Schizophyllum commune | ferulic acid + ? | - |
? | |
| ferulic acid methyl ester + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Neocallimastix sp. | ferulic acid + methanol | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Schizophyllum commune | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Neocallimastix sp. | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| O-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | PAXX | Neocallimastix sp. | p-coumaric acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| O-beta-D-xylopyranosyl-(1-4)-O-[5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAX3-II | Neocallimastix sp. | ferulic acid + O-beta-D-xylopyranosyl-(1-4)-[O-alpha-L-arabinofuranosyl-(1-3)]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Streptomyces olivochromogenes |
| monomer | 1 * 68000, SDS-PAGE, FAE-I | Neocallimastix sp. |
| monomer | 1 * 24000, SDS-PAGE, FAE-II | Neocallimastix sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
both FAE-I and FAE-II relatively stable | Neocallimastix sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Neocallimastix sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.8 | FAE-I most active in this range | Neocallimastix sp. |
| 6.4 | 7.6 | FAE-II most active in this range | Neocallimastix sp. |
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