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Literature summary for 3.1.1.73 extracted from
- The purification and characterization of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes (1991), J. Gen. Microbiol., 137, 2339-2345.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.86 | - |
methyl ferulate | - |
Streptomyces olivochromogenes |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces olivochromogenes | - |
- |
| extracellular | - |
Neocallimastix sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Streptomyces olivochromogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Schizophyllum commune | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes NRCC 2258 | - |
ferulate + polysaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Neocallimastix sp. | - |
rumen fungus, MC-2 | - |
| Schizophyllum commune | - |
- |
- |
| Streptomyces olivochromogenes | - |
NRCC 2258 | - |
| Streptomyces olivochromogenes NRCC 2258 | - |
NRCC 2258 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces olivochromogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.19 | - |
methyl ferulate as substrate | Streptomyces olivochromogenes |
| 1.51 | - |
de-starched wheat bran as substrate | Streptomyces olivochromogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification | Streptomyces olivochromogenes |
| 4°C, pH 7, enzyme loses activity over 24 h even in presence of EDTA, DTT, Mg2+ and Ca2+, additives which stabilizes the enzyme during purification | Streptomyces olivochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cinnamic acid methyl ester + H2O | - |
Aspergillus niger | cinnamic acid + methanol | - |
? | |
| cinnamic acid methyl ester + H2O | - |
Neocallimastix sp. | cinnamic acid + methanol | - |
? | |
| de-starched wheat bran + H2O | - |
Aspergillus niger | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | purified enzyme releases ferulic acid only in presence of xylanase | Streptomyces olivochromogenes | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | purified enzyme releases ferulic acid only in presence of xylanase | Schizophyllum commune | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | purified enzyme releases ferulic acid only in presence of xylanase | Streptomyces olivochromogenes NRCC 2258 | ferulic acid + ? | - |
? | |
| ferulic acid methyl ester + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Neocallimastix sp. | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes NRCC 2258 | ferulic acid + methanol | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Schizophyllum commune | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes NRCC 2258 | ferulate + polysaccharide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Neocallimastix sp. |
| monomer | 1 * 29000, SDS-PAGE | Streptomyces olivochromogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-hydroxy-3-methoxycinnamic acid esterase | - |
Streptomyces olivochromogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
methyl ferulate as substrate | Streptomyces olivochromogenes |
| 50 | - |
- |
Neocallimastix sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | - |
Aspergillus niger |
| 20 | 70 | - |
Streptomyces olivochromogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
methyl ferulate as substrate | Streptomyces olivochromogenes |
| 7.5 | - |
- |
Neocallimastix sp. |
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