Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli cytosol and periplasm as 2 active forms due to cleavage at 2 different sites within the signal peptide | Orpinomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
4-nitrophenyl acetate | pH 6.7, 37°C | Orpinomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Orpinomyces sp. | O59869 | gene axeA | - |
Purification (Comment) | Organism |
---|---|
2 recombinant enzyme forms from Escherichia coli, over 30fold | Orpinomyces sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. | Orpinomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
purified recombinant enzyme, tri-O-acetyl-D-galactal | Orpinomyces sp. |
0.2 | - |
purified recombinant enzyme, xylose tetraacetate | Orpinomyces sp. |
0.31 | - |
purified recombinant enzyme, glucose pentaacetate | Orpinomyces sp. |
0.49 | - |
purified recombinant enzyme, 4-nitrophenyl acetate | Orpinomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Orpinomyces sp. | 4-nitrophenol + acetate | - |
? | |
acetylated xylan + H2O | the enzyme acts synergistically with xylanase to degrade acetylated xylan | Orpinomyces sp. | xylan + acetate | - |
? | |
glucose pentaacetate + H2O | - |
Orpinomyces sp. | glucose + acetate | - |
? | |
tri-O-acetyl-D-galactal + H2O | - |
Orpinomyces sp. | D-galactal + acetate | - |
? | |
xylose tetraacetate + H2O | - |
Orpinomyces sp. | xylose + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000-40000, recombinant enzyme, SDS-PAGE | Orpinomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
acetyl xylan esterase | - |
Orpinomyces sp. |
AxeA | - |
Orpinomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Orpinomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Orpinomyces sp. |