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Literature summary for 3.1.1.7 extracted from

  • de Assis, C.; Linhares, A.; Oliveira, V.; Fran็a, R.; Santos, J.; Marcuschi, M.; Maciel Carvalho, E.; Bezerra, R.; Carvalho Jr., L.
    Characterization of catalytic efficiency parameters of brain cholinesterases in tropical fish (2014), Fish Physiol. Biochem., 40, 1659-1668.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Arapaima gigas
BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Colossoma macropomum
BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Oreochromis niloticus
BW284c51 i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide Rachycentron canadum
eserine
-
Arapaima gigas
eserine
-
Colossoma macropomum
eserine
-
Oreochromis niloticus
eserine
-
Rachycentron canadum
additional information tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) Colossoma macropomum
additional information tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) Oreochromis niloticus
additional information tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) Rachycentron canadum
neostigmine bromide
-
Arapaima gigas
neostigmine bromide
-
Colossoma macropomum
neostigmine bromide
-
Oreochromis niloticus
neostigmine bromide
-
Rachycentron canadum
tetraisopropyl diphosphoramide
-
Arapaima gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
0.42
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
0.43
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
0.43
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
0.63
-
propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
1.02
-
propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
1.61
-
butyrylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
2.24
-
propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
2.9
-
propionylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
4.23
-
butyrylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
5.14
-
butyrylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
244000
-
x * 244000, SDS-PAGE Colossoma macropomum
244000
-
x * 244000, SDS-PAGE Rachycentron canadum
252000
-
x * 252000, SDS-PAGE Oreochromis niloticus
252000
-
x * 252000, SDS-PAGE Colossoma macropomum
287000
-
x * 287000, SDS-PAGE Colossoma macropomum
299000
-
x * 299000, SDS-PAGE Colossoma macropomum
299000
-
x * 299000, SDS-PAGE Arapaima gigas

Organism

Organism UniProt Comment Textmining
Arapaima gigas
-
-
-
Colossoma macropomum
-
-
-
Oreochromis niloticus
-
-
-
Rachycentron canadum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Oreochromis niloticus
-
brain
-
Colossoma macropomum
-
brain
-
Rachycentron canadum
-
brain
-
Arapaima gigas
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine + H2O most efficiently hydrolyzed substrate Oreochromis niloticus acetate + thiocholine
-
?
acetylthiocholine + H2O most efficiently hydrolyzed substrate Colossoma macropomum acetate + thiocholine
-
?
acetylthiocholine + H2O most efficiently hydrolyzed substrate Rachycentron canadum acetate + thiocholine
-
?
acetylthiocholine + H2O most efficiently hydrolyzed substrate Arapaima gigas acetate + thiocholine
-
?
butyrylthiocholine + H2O
-
Oreochromis niloticus butyrate + thiocholine
-
?
butyrylthiocholine + H2O
-
Colossoma macropomum butyrate + thiocholine
-
?
butyrylthiocholine + H2O
-
Rachycentron canadum butyrate + thiocholine
-
?
butyrylthiocholine + H2O
-
Arapaima gigas butyrate + thiocholine
-
?
propionylthiocholine + H2O
-
Oreochromis niloticus propanoate + thiocholine
-
?
propionylthiocholine + H2O
-
Colossoma macropomum propanoate + thiocholine
-
?
propionylthiocholine + H2O
-
Rachycentron canadum propanoate + thiocholine
-
?
propionylthiocholine + H2O
-
Arapaima gigas propanoate + thiocholine
-
?

Subunits

Subunits Comment Organism
? x * 252000, SDS-PAGE Oreochromis niloticus
? x * 252000, SDS-PAGE Colossoma macropomum
? x * 244000, SDS-PAGE Colossoma macropomum
? x * 244000, SDS-PAGE Rachycentron canadum
? x * 287000, SDS-PAGE Colossoma macropomum
? x * 299000, SDS-PAGE Colossoma macropomum
? x * 299000, SDS-PAGE Arapaima gigas

Synonyms

Synonyms Comment Organism
AChE
-
Oreochromis niloticus
AChE
-
Colossoma macropomum
AChE
-
Rachycentron canadum
AChE
-
Arapaima gigas

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.04
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
1.29
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
1.55
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
1.84
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000009
-
neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
0.000001
-
neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
0.000002
-
neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
0.0000021
-
eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
0.0000025
-
eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
0.000004
-
neostigmine bromide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
0.0000045
-
eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
0.0000055
-
eserine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
0.00002
-
BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
0.000045
-
BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum
0.00005
-
BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
0.00219
-
BW284c51 in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
0.00219
-
tetraisopropyl diphosphoramide in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00015
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum neostigmine bromide
0.00016
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus neostigmine bromide
0.00017
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum neostigmine bromide
0.00031
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas eserine
0.000371
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum eserine
0.00049
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum eserine
0.00059
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas neostigmine bromide
0.00076
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus eserine
0.00396
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum BW284c51
0.00412
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus BW284c51
0.00752
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum BW284c51
0.3184
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas BW284c51
0.3184
-
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas tetraisopropyl diphosphoramide

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23000
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Colossoma macropomum
24700
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Arapaima gigas
39700
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Oreochromis niloticus
42000
-
acetylthiocholine in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication Rachycentron canadum