Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BW284c51 | i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | Arapaima gigas | |
BW284c51 | i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | Colossoma macropomum | |
BW284c51 | i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | Oreochromis niloticus | |
BW284c51 | i.e. 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | Rachycentron canadum | |
eserine | - |
Arapaima gigas | |
eserine | - |
Colossoma macropomum | |
eserine | - |
Oreochromis niloticus | |
eserine | - |
Rachycentron canadum | |
additional information | tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) | Colossoma macropomum | |
additional information | tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) | Oreochromis niloticus | |
additional information | tetraisopropyl diphosphoramide does not reach 50% inhibition in the concentration range of the assays (0.001-10 mM) | Rachycentron canadum | |
neostigmine bromide | - |
Arapaima gigas | |
neostigmine bromide | - |
Colossoma macropomum | |
neostigmine bromide | - |
Oreochromis niloticus | |
neostigmine bromide | - |
Rachycentron canadum | |
tetraisopropyl diphosphoramide | - |
Arapaima gigas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | |
0.42 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | |
0.43 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
0.43 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | |
0.63 | - |
propionylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
1.02 | - |
propionylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | |
1.61 | - |
butyrylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
2.24 | - |
propionylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | |
2.9 | - |
propionylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | |
4.23 | - |
butyrylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | |
5.14 | - |
butyrylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
244000 | - |
x * 244000, SDS-PAGE | Colossoma macropomum |
244000 | - |
x * 244000, SDS-PAGE | Rachycentron canadum |
252000 | - |
x * 252000, SDS-PAGE | Oreochromis niloticus |
252000 | - |
x * 252000, SDS-PAGE | Colossoma macropomum |
287000 | - |
x * 287000, SDS-PAGE | Colossoma macropomum |
299000 | - |
x * 299000, SDS-PAGE | Colossoma macropomum |
299000 | - |
x * 299000, SDS-PAGE | Arapaima gigas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arapaima gigas | - |
- |
- |
Colossoma macropomum | - |
- |
- |
Oreochromis niloticus | - |
- |
- |
Rachycentron canadum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Oreochromis niloticus | - |
brain | - |
Colossoma macropomum | - |
brain | - |
Rachycentron canadum | - |
brain | - |
Arapaima gigas | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | most efficiently hydrolyzed substrate | Oreochromis niloticus | acetate + thiocholine | - |
? | |
acetylthiocholine + H2O | most efficiently hydrolyzed substrate | Colossoma macropomum | acetate + thiocholine | - |
? | |
acetylthiocholine + H2O | most efficiently hydrolyzed substrate | Rachycentron canadum | acetate + thiocholine | - |
? | |
acetylthiocholine + H2O | most efficiently hydrolyzed substrate | Arapaima gigas | acetate + thiocholine | - |
? | |
butyrylthiocholine + H2O | - |
Oreochromis niloticus | butyrate + thiocholine | - |
? | |
butyrylthiocholine + H2O | - |
Colossoma macropomum | butyrate + thiocholine | - |
? | |
butyrylthiocholine + H2O | - |
Rachycentron canadum | butyrate + thiocholine | - |
? | |
butyrylthiocholine + H2O | - |
Arapaima gigas | butyrate + thiocholine | - |
? | |
propionylthiocholine + H2O | - |
Oreochromis niloticus | propanoate + thiocholine | - |
? | |
propionylthiocholine + H2O | - |
Colossoma macropomum | propanoate + thiocholine | - |
? | |
propionylthiocholine + H2O | - |
Rachycentron canadum | propanoate + thiocholine | - |
? | |
propionylthiocholine + H2O | - |
Arapaima gigas | propanoate + thiocholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 252000, SDS-PAGE | Oreochromis niloticus |
? | x * 252000, SDS-PAGE | Colossoma macropomum |
? | x * 244000, SDS-PAGE | Colossoma macropomum |
? | x * 244000, SDS-PAGE | Rachycentron canadum |
? | x * 287000, SDS-PAGE | Colossoma macropomum |
? | x * 299000, SDS-PAGE | Colossoma macropomum |
? | x * 299000, SDS-PAGE | Arapaima gigas |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Oreochromis niloticus |
AChE | - |
Colossoma macropomum |
AChE | - |
Rachycentron canadum |
AChE | - |
Arapaima gigas |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.04 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | |
1.29 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
1.55 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | |
1.84 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000009 | - |
neostigmine bromide | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | |
0.000001 | - |
neostigmine bromide | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
0.000002 | - |
neostigmine bromide | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | |
0.0000021 | - |
eserine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | |
0.0000025 | - |
eserine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
0.000004 | - |
neostigmine bromide | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | |
0.0000045 | - |
eserine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | |
0.0000055 | - |
eserine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | |
0.00002 | - |
BW284c51 | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | |
0.000045 | - |
BW284c51 | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | |
0.00005 | - |
BW284c51 | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
0.00219 | - |
BW284c51 | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | |
0.00219 | - |
tetraisopropyl diphosphoramide | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00015 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | neostigmine bromide | |
0.00016 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | neostigmine bromide | |
0.00017 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | neostigmine bromide | |
0.00031 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | eserine | |
0.000371 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | eserine | |
0.00049 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | eserine | |
0.00059 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | neostigmine bromide | |
0.00076 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | eserine | |
0.00396 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum | BW284c51 | |
0.00412 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | BW284c51 | |
0.00752 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | BW284c51 | |
0.3184 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | BW284c51 | |
0.3184 | - |
in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | tetraisopropyl diphosphoramide |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23000 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Colossoma macropomum | |
24700 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Arapaima gigas | |
39700 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Oreochromis niloticus | |
42000 | - |
acetylthiocholine | in 0.5 M Tris-HCl buffer, pH 8.0, temperature not specified in the publication | Rachycentron canadum |