Cloned (Comment) | Organism |
---|---|
expression analysis by semi-quantitative PCR, sequence comparisons, subcloning of carp AChE in Escherichia coli strain DH5alpha, and expression of wild-type and mutant enzymes in Pichia pastoris strain X-33 | Cyprinus carpio |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of two mutant enzymes, one lacking the T peptide and the second lacking the T peptide and containing the alpha-factor prepro signal sequence from Saccharomyces cerevisiae in place of the original signal sequence | Cyprinus carpio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetylthiocholine | the recombinant carp AChE shows substrate inhibition at high substrate concentrations of acetyl- and propionylthiocholine | Cyprinus carpio | |
acetylthiocholine iodide | - |
Cyprinus carpio | |
butyrylthiocholine | - |
Cyprinus carpio | |
butyrylthiocholine iodide | - |
Cyprinus carpio | |
BW284c51 | - |
Cyprinus carpio | |
diisopropylfluorophosphate | - |
Cyprinus carpio | |
edrophonium | - |
Cyprinus carpio | |
ethopropazine | low inhibition | Cyprinus carpio | |
Phenylmethylsulfonylfluoride | - |
Cyprinus carpio | |
procainamide | low inhibition | Cyprinus carpio | |
propionylthiocholine | the recombinant carp AChE shows substrate inhibition at high substrate concentrations of acetyl- and propionylthiocholine | Cyprinus carpio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000232 | - |
acetylthiocholine iodide | pH 7.6, 40°C, native enzyme | Cyprinus carpio | |
0.00041 | - |
butyrylthiocholine iodide | pH 7.6, 40°C, native enzyme | Cyprinus carpio | |
0.000526 | - |
propionylthiocholine iodide | pH 7.6, 40°C, native enzyme | Cyprinus carpio | |
0.00205 | - |
acetylthiocholine iodide | pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | |
0.00605 | - |
propionylthiocholine iodide | pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | |
0.109 | - |
butyrylthiocholine iodide | pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE | Cyprinus carpio |
54000 | - |
x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE | Cyprinus carpio |
73000 | - |
x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE | Cyprinus carpio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyprinus carpio | B7X755 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme is N-glycosylated, and the sequence contains N-glycosylation consensus sites | Cyprinus carpio |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes 276fold from Pichia pastoris strain X-33 culture supernatant by anion exchange and edrophonium adsorption chromatography, followed by gel filtration | Cyprinus carpio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Cyprinus carpio | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42 | - |
mutant enzyme lacking the T peptide and possessing a Saccharomyces cerevisiae alpha-factor prepro signal sequence in recombinant Pichia pastoris cells | Cyprinus carpio |
176 | - |
wild-type enzyme in recombinant Pichia pastoris cells | Cyprinus carpio |
181 | - |
mutant enzyme lacking the T peptide in recombinant Pichia pastoris cells | Cyprinus carpio |
4970 | - |
purified recombinant wild-type enzyme | Cyprinus carpio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyrylthiocholine iodide + H2O | - |
Cyprinus carpio | thiocholine iodide + butyrate | - |
? | |
propionylthiocholine iodide + H2O | - |
Cyprinus carpio | thiocholine iodide + propionate | - |
? | |
S-acetylthiocholine iodide + H2O | detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB, the enzyme contains a Ser, Glu, and His catalytic triad, overview | Cyprinus carpio | acetate + thiocholine iodide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73000 + x * 54000 + x * 22000, recombinant enzyme, SDS-PAGE | Cyprinus carpio |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Cyprinus carpio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Cyprinus carpio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Cyprinus carpio |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
acetylthiocholine iodide | pH 7.6, 40°C, native enzyme | Cyprinus carpio | |
0.175 | - |
acetylthiocholine iodide | pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | |
0.418 | - |
butyrylthiocholine iodide | pH 7.6, 40°C, native enzyme | Cyprinus carpio |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00217 | - |
pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | ethopropazine | |
0.00294 | - |
pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | Phenylmethylsulfonylfluoride | |
0.00305 | - |
pH 7.6, 40°C, native enzyme | Cyprinus carpio | Phenylmethylsulfonylfluoride | |
0.00335 | - |
pH 7.6, 40°C, native enzyme | Cyprinus carpio | ethopropazine | |
0.00347 | - |
pH 7.6, 40°C, native enzyme | Cyprinus carpio | procainamide | |
0.00356 | - |
pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | procainamide | |
0.00439 | - |
pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | diisopropylfluorophosphate | |
0.00441 | - |
pH 7.6, 40°C, native enzyme | Cyprinus carpio | diisopropylfluorophosphate | |
0.00522 | - |
pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | edrophonium | |
0.0066 | - |
pH 7.6, 40°C, native enzyme | Cyprinus carpio | edrophonium | |
0.00682 | - |
pH 7.6, 40°C, recombinant enzyme | Cyprinus carpio | BW284c51 | |
0.00722 | - |
pH 7.6, 40°C, native enzyme | Cyprinus carpio | BW284c51 |