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Literature summary for 3.1.1.7 extracted from
- Characterization of trimeric acetylcholinesterase from a legume plant, Macroptilium atropurpureum Urb. (2008), Planta, 227, 809-822.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determnination and anaylsis, phylogenetic tree | Macroptilium atropurpureum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetylthiocholine | substrate inhibition | Macroptilium atropurpureum |  |
| neostigmine bromide | weak inhibition | Macroptilium atropurpureum | |
| propionylthiocholine | substrate inhibition | Macroptilium atropurpureum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
propionylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
| 0.08 | - |
acetylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
| 1.9 | - |
acetylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
| 2 | - |
propionylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 121000 | - |
gel filtration | Macroptilium atropurpureum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine chloride + H2O | Macroptilium atropurpureum | - |
choline chloride + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Macroptilium atropurpureum | - |
siratro | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains three potential N-glycosylation sites | Macroptilium atropurpureum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 134.8fold from seedlings by ammonium sulfate fractionation, gel filtration, dialysis, anion exchange chromatography, and again gel filtration to homogeneity | Macroptilium atropurpureum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Macroptilium atropurpureum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Macroptilium atropurpureum |
| 0.957 | - |
purified native enzyme | Macroptilium atropurpureum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine chloride + H2O | - |
Macroptilium atropurpureum | choline chloride + acetate | - |
? | |
| acetylthiocholine chloride + H2O | - |
Macroptilium atropurpureum | thiocholine chloride + acetate | - |
? | |
| propionylcholine chloride + H2O | - |
Macroptilium atropurpureum | choline chloride + propionate | - |
? | |
| propionylthiocholine chloride + H2O | - |
Macroptilium atropurpureum | thiocholine chloride + propionate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 41000-42000, SDS-PAGE, 3 * 40597.21, sequence calculation | Macroptilium atropurpureum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AChE | - |
Macroptilium atropurpureum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Macroptilium atropurpureum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
acetylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
| 0.5 | - |
propionylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
| 1.2 | - |
acetylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
| 1.2 | - |
propionylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Macroptilium atropurpureum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Macroptilium atropurpureum | sequence calculation | - |
6.16 |
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